Anti-SOS1 Antibody (CAB3272)

Antibody Name:	Anti-SOS1 Antibody
Antibody SKU:	CAB3272
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1034-1333 of human SOS1 (NP_005624.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse liver, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1034-1333 of human SOS1 (NP_005624.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PLKS PGVR PSNP RPGT MRHP TPLQ QEPR KISY SRIP ESET ESTA SAPN SPRT PLTP PPAS GASS TTDV CSVF DSDH SSPF HSSN DTVF IQVT LPHG PRSA SVSS ISLT KGTD EVPV PPPV PPRR RPES APAE SSPS KIMS KHLD SPPA IPPR QPTS KAYS PRYS ISDR TSIS DPPE SPPL LPPR EPVR TPDV FSSS PLHL QPPP LGKK SDHG NAFF PNSP SPFT PPPP QTPS PHGT RRHL PSPP LTQE VDLH SIAG PPVP PRQS TSQH IPKL PPKT YKRE HTHP SMHR DGPP LLEN AHSS
Gene ID:	6654
Uniprot:	Q07889
Cellular Location:
Calculated MW:	36kDa/152kDa
Observed MW:	175kDa/200kDa

Synonyms:

SOS1, GF1, GGF1, GINGF, HGF, NS4

Background:

This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4.

UniProt Protein Function:	Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity.
NCBI Summary:	This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008]
UniProt Code:	Q07889
NCBI GenInfo Identifier:	6094322
NCBI Gene ID:	6654
NCBI Accession:	Q07889.1
UniProt Secondary Accession:	Q07889,A8K2G3, B4DXG2,
UniProt Related Accession:	Q07889
Molecular Weight:
NCBI Full Name:	Son of sevenless homolog 1
NCBI Synonym Full Names:	SOS Ras/Rac guanine nucleotide exchange factor 1
NCBI Official Symbol:	SOS1
NCBI Official Synonym Symbols:	GF1; HGF; NS4; GGF1; GINGF; SOS-1
NCBI Protein Information:	son of sevenless homolog 1
UniProt Protein Name:	Son of sevenless homolog 1
Protein Family:	Son of sevenless
UniProt Gene Name:	SOS1

	Western blot analysis of extracts of various cell lines, using SOS1 antibody (CAB3272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of rat brain using SOS1 antibody (CAB3272) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat heart using SOS1 antibody (CAB3272) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
SOS1 Antibody (PACO12397)	Human SOS1 (Son of sevenless homolog 1) ELISA Kit (HUFI06402)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)