The SRSF6 Antibody (CAB14603) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is suitable for Western blot applications. It binds to the SRSF6 protein, allowing for the detection and analysis of SRSF6 in a variety of cell types, making it ideal for investigations in molecular biology and cancer research.SRSF6, also known as SRp55, plays a crucial role in alternative splicing, which is essential for generating protein diversity and regulating gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SRSF6 Antibody
SKU:
CAB14603
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B52, SFRS6, SRP55, HEL-S-91, SRSF6
Positive Sample:
HT-29, K-562, HeLa, HepG2, Mouse lung
Cellular Localization:
Nucleus, Nucleus Speckle.
Calculated MW:
40kDa
Observed MW:
55kDa
The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. In addition, two pseudogenes, one on chromosome 17 and the other on the X chromosome, have been found for this gene.
Purification Method
Affinity purification
Gene ID
6431
RRID
AB_2761479
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SRSF6 Rabbit pAb (CAB14603) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Mouse lung, using SRSF6 Rabbit pAb (CAB14603) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
