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Anti-ST3GAL1 Antibody (CAB11616)

SKU:
CAB11616
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-ST3GAL1 Antibody
Antibody SKU:CAB11616
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-135 of human ST3GAL1 (NP_003024.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-135 of human ST3GAL1 (NP_003024.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TTWF PKQM VLEL SENL KRLI KHRP CTCT HCIG QRKL SAWF DERF NQTM QPLL TAQN ALLE DDTY RWWL RLQR EKKP NNLN DTIK ELFR VVPG NVDP MLEK R
Gene ID:6482
Uniprot:Q11201
Cellular Location:Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein
Calculated MW:39kDa
Observed MW:39kDa
Synonyms:ST3GAL1, Gal-NAc6S, SIAT4A, SIATFL, ST3GalA, ST3GalA.1, ST3GalIA, 1, ST3O
Background:The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet.
UniProt Protein Function:ST3GAL1: It may be responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O- linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values. Belongs to the glycosyltransferase 29 family.
UniProt Protein Details:

Protein type:EC 2.4.99.4; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Glycan Metabolism - keratan sulfate biosynthesis; Transferase

Chromosomal Location of Human Ortholog: 8q24.22

Cellular Component: Golgi membrane; membrane; integral to Golgi membrane

Molecular Function:beta-galactoside alpha-2,3-sialyltransferase activity

Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; glycosaminoglycan metabolic process; dolichol-linked oligosaccharide biosynthetic process; O-glycan processing; keratan sulfate biosynthetic process; carbohydrate metabolic process; protein amino acid N-linked glycosylation; protein modification process; pathogenesis; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; protein amino acid phosphorylation; N-acetylneuraminate metabolic process

NCBI Summary:The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq, Jul 2008]
UniProt Code:Q11201
NCBI GenInfo Identifier:1705559
NCBI Gene ID:6482
NCBI Accession:Q11201.1
UniProt Secondary Accession:Q11201,O60677, Q9UN51,
UniProt Related Accession:Q11201
Molecular Weight:39,075 Da
NCBI Full Name:CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
NCBI Synonym Full Names:ST3 beta-galactoside alpha-2,3-sialyltransferase 1
NCBI Official Symbol:ST3GAL1  
NCBI Official Synonym Symbols:ST3O; SIAT4A; SIATFL; ST3GalA; ST3GalIA; Gal-NAc6S; ST3GalA.1; ST3GalIA,1  
NCBI Protein Information:CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1; SIAT4-A; ST3GalI; alpha 2,3-ST 1; beta-galactoside alpha-2,3-sialyltransferase 1; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; sialyltransferase 4A (beta-galactoside alpha-2,3-sialytransferase); sialyltransferase 4A (beta-galactoside alpha-2,3-sialyltransferase); sialyltransferase 4A (beta-galactosidase alpha-2,3-sialytransferase)
UniProt Protein Name:CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
UniProt Synonym Protein Names:Gal-NAc6S; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; SIATFL; ST3Gal I; ST3GalI; ST3GalA.1; ST3O; Sialyltransferase 4A; SIAT4-A
Protein Family:CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
UniProt Gene Name:ST3GAL1  
UniProt Entry Name:SIA4A_HUMAN
Anti-ST3GAL1 Antibody (CAB11616)Western blot analysis of extracts of mouse liver, using ST3GAL1 antibody (CAB11616) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.

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