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Anti-ST8SIA1 Antibody (CAB9648)

SKU:
CAB9648
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
€139 - €419
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Description

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Antibody Name:Anti-ST8SIA1 Antibody
Antibody SKU:CAB9648
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 49-270 of human ST8SIA1 (NP_003025.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, SGC-7901
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 49-270 of human ST8SIA1 (NP_003025.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YRLP NEKE IVQG VLQQ GTAW RRNQ TAAR AFRK QMED CCDP AHLF AMTK MNSP MGKS MWYD GEFL YSFT IDNS TYSL FPQA TPFQ LPLK KCAV VGNG GILK KSGC GRQI DEAN FVMR CNLP PLSS EYTK DVGS KSQL VTAN PSII RQRF QNLL WSRK TFVD NMKI YNHS YIYM PAFS MKTG TEPS LRVY YTLS DVGA NQTV LFAN PNFL RSIG KFWK SRGI HA
Gene ID:6489
Uniprot:Q92185
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:15kDa/40kDa
Observed MW:40kDa
Synonyms:ST8SIA1, GD3S, SIAT8, SIAT8-A, SIAT8A, ST8SiaI
Background:Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:ST8SIA1: Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid. Belongs to the glycosyltransferase 29 family.
UniProt Protein Details:

Protein type:Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; EC 2.4.99.8; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 12p12.1-p11.2

Cellular Component: Golgi membrane; integral to membrane; integral to Golgi membrane

Molecular Function:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity; sialyltransferase activity

Biological Process: glycosphingolipid biosynthetic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; positive regulation of cell proliferation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

NCBI Summary:Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. [provided by RefSeq, Jul 2008]
UniProt Code:Q92185
NCBI GenInfo Identifier:2494829
NCBI Gene ID:6489
NCBI Accession:Q92185.1
UniProt Secondary Accession:Q92185,Q17RL0, Q6PZN5, Q93064, A8K4H6,
UniProt Related Accession:Q92185
Molecular Weight:15,504 Da
NCBI Full Name:Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
NCBI Synonym Full Names:ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
NCBI Official Symbol:ST8SIA1  
NCBI Official Synonym Symbols:GD3S; SIAT8; SIAT8A; SIAT8-A; ST8SiaI  
NCBI Protein Information:alpha-N-acetylneuraminide alpha-2,8-sialyltransferase; ganglioside GD3 synthase; ganglioside GT3 synthase; sialytransferase St8Sia I; sialyltransferase St8Sia I; alpha-2,8-sialyltransferase 8A; disialoganglioside (GD3) synthase; ganglioside-specific alpha-2,8-polysialyltransferase; sialyltransferase 8 (alpha-N-acetylneuraminate: alpha-2,8-sialytransferase, GD3 synthase) A; sialyltransferase 8A (alpha-N-acetylneuraminate: alpha-2,8-sialyltransferase, GD3 synthase)
UniProt Protein Name:Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
UniProt Synonym Protein Names:Alpha-2,8-sialyltransferase 8A; Ganglioside GD3 synthase; Ganglioside GT3 synthase; Sialyltransferase 8A; SIAT8-A; Sialyltransferase St8Sia I; ST8SiaI
Protein Family:Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
UniProt Gene Name:ST8SIA1  
UniProt Entry Name:SIA8A_HUMAN
Anti-ST8SIA1 Polyclonal Antibody (CAB9648)Western blot analysis of extracts of various cell lines, using ST8SIA1 antibody (CAB9648) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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