The STAR Antibody (CAB16432) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the STAR protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and gene regulation studies.The STAR protein, also known as Signal Transduction Activator of RNA, plays a crucial role in controlling the translation of specific mRNAs, ultimately influencing protein synthesis and cellular function.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STAR Antibody
SKU:
CAB16432
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STARD1, StAR
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Mitochondrion.
Calculated MW:
32kDa
Observed MW:
28kDa
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.
Purification Method
Affinity purification
Gene ID
6770
RRID
AB_2772418
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using StAR Rabbit pAb (CAB16432) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Confocal immunofluorescence analysis of mouse adrenal gland using StAR Rabbit pAb (CAB16432) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded mouse adrenal gland using StAR Rabbit pAb (CAB16432) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using StAR Rabbit pAb (CAB16432) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
