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Anti-Symmetric DiMethyl-Histone H3-R8 Antibody (CAB2374)

SKU:
CAB2374
Product Type:
Antibody
Applications:
WB
IHC
IF
IP
ChIP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R8 Antibody CAB2374
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R8 Antibody CAB2374
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Symmetric DiMethyl-Histone H3-R8 Antibody CAB2374
€139 - €419
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Description

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Anti-Symmetric DiMethyl-Histone H3-R8 Antibody (CAB2374)

The Symmetric Dimethyl Histone H3 R8 Polyclonal Antibody (CAB2374) is a valuable tool for researchers studying histone modifications and epigenetic regulation. This antibody, produced in rabbits, specifically targets the histone H3 protein with symmetric dimethylation at arginine 8 (H3R8me2s). Histone modifications, such as symmetric dimethylation of H3R8, play a crucial role in regulating gene expression and chromatin structure. The CAB2374 antibody is highly reactive with human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of H3R8me2s levels in various cell types and experimental conditions.

Research on histone modifications like symmetric dimethylation of H3R8 is essential for understanding how epigenetic changes influence cellular processes, development, and diseases such as cancer and neurodegenerative disorders. By studying the role of H3R8me2s in gene regulation and chromatin dynamics, researchers can gain insights into the mechanisms underlying disease progression and potentially identify new therapeutic targets.

Antibody Name:Anti-Symmetric DiMethyl-Histone H3-R8 Antibody
Antibody SKU:CAB2374
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP ChIP ChIPseq
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3
Application:WB IHC IF IP ChIP ChIPseq
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:50 ChIPseq 1:20 - 1:50
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:NIH/3T3
Immunogen:A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8290
Uniprot:Q16695
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:17kDa
Synonyms:H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
UniProt Protein Function:HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:Q16695
NCBI GenInfo Identifier:18202512
NCBI Gene ID:8290
NCBI Accession:Q16695.3
UniProt Secondary Accession:Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:Q16695
Molecular Weight:
NCBI Full Name:Histone H3.1t
NCBI Synonym Full Names:histone cluster 3, H3
NCBI Official Symbol:HIST3H3  
NCBI Official Synonym Symbols:H3t; H3.4; H3/g; H3FT  
NCBI Protein Information:histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:Histone H3.1t
UniProt Synonym Protein Names:H3/g
UniProt Gene Name:HIST3H3  
UniProt Entry Name:H31T_HUMAN
Western blot - Symmetric DiMethyl-Histone H3-R8 Rabbit pAb Western blot analysis of extracts of NIH/3T3 cells, using Symmetric DiMethyl-Histone H3-R8 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
- Symmetric DiMethyl-Histone H3-R8 Rabbit pAb Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R8 antibody (A2374).
Immunofluorescence - Symmetric DiMethyl-Histone H3-R8 Rabbit pAb Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R8 antibody (A2374). Blue: DAPI for nuclear staining.

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