Anti-SYP Antibody (CAB6344)

Antibody Name:	Anti-SYP Antibody
Antibody SKU:	CAB6344
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SYP (NP_003170.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Mouse brain, Mouse eye, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SYP (NP_003170.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AFMW LVSS SAWA KGLS DVKM ATDP ENII KEMP VCRQ TGNT CKEL RDPV TSGL NTSV VFGF LNLV LWVG NLWF VFKE TGWA APFL RAPP GAPE KQPA PGDA Y
Gene ID:	6855
Uniprot:	P08247
Cellular Location:	Cell junction, Cytoplasmic vesicle, Multi-pass membrane protein, secretory vesicle, synapse, synaptic vesicle membrane, synaptosome
Calculated MW:	20kDa/33kDa
Observed MW:	38kDa

Synonyms:	MRX96, MRXSYP, SYP
Background:	This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).

UniProt Protein Function:	SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: Xp11.23-p11.22 Cellular Component: cell junction; excitatory synapse; integral to synaptic vesicle membrane; neuron projection; presynaptic active zone; presynaptic membrane; synaptic vesicle; synaptic vesicle membrane; terminal button Molecular Function:cholesterol binding; identical protein binding; protein self-association; SH2 domain binding; transporter activity Biological Process: endocytosis; regulation of long-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity; regulation of short-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis Disease: Mental Retardation, X-linked 96
NCBI Summary:	This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
UniProt Code:	P08247
NCBI GenInfo Identifier:	135162
NCBI Gene ID:	6855
NCBI Accession:	P08247.3
UniProt Secondary Accession:	P08247,Q6P2F7, B2R7L6, B7Z359,
UniProt Related Accession:	P08247
Molecular Weight:	20,757 Da
NCBI Full Name:	Synaptophysin
NCBI Synonym Full Names:	synaptophysin
NCBI Official Symbol:	SYP
NCBI Official Synonym Symbols:	MRX96; MRXSYP
NCBI Protein Information:	synaptophysin
UniProt Protein Name:	Synaptophysin
UniProt Synonym Protein Names:	Major synaptic vesicle protein p38
Protein Family:	Synaptophysin
UniProt Gene Name:	SYP
UniProt Entry Name:	SYPH_HUMAN

	Western blot analysis of extracts of various cell lines, using SYP antibody (CAB6344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded mouse pancreas using SYP Rabbit pAb (CAB6344) at dilution of 100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat pancreas using SYP Rabbit pAb (CAB6344) at dilution of 100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using SYP Rabbit pAb (CAB6344) at dilution of 100 (40x lens).
	Immunofluorescence analysis of C6 cells using SYP Rabbit pAb (CAB6344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Synaptophysin Antibody (CAB19122)	Human Synaptophysin ELISA Kit
SYP Antibody (PACO01573)	Human Synaptophysin (SYP) ELISA Kit
SYP Antibody (PACO12606)	Synaptophysin Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)