Anti-TAP2 Antibody (CAB1610)

Antibody Name:	Anti-TAP2 Antibody
Antibody SKU:	CAB1610
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 430-680 of human TAP2 (NP_000535.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	CEM, MCF7, Mouse spleen, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 430-680 of human TAP2 (NP_000535.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YGDM LSNV GAAE KVFS YMDR QPNL PSPG TLAP TTLQ GVVK FQDV SFAY PNRP DRPV LKGL TFTL RPGE VTAL VGPN GSGK STVA ALLQ NLYQ PTGG QVLL DEKP ISQY EHCY LHSQ VVSV GQEP VLFS GSVR NNIA YGLQ SCED DKVM AAAQ AAHA DDFI QEME HGIY TDVG EKGS QLAA GQKQ RLAI ARAL VRDP RVLI LDEA TSAL DVQC EQAL QDWN SRGD RTVL VIAH RLQA VQRA HQIL VLQE GKL
Gene ID:	6891
Uniprot:	Q03519
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	72kDa/75kDa
Observed MW:	76kDa

Synonyms:

TAP2, ABC18, ABCB3, APT2, D6S217E, PSF-2, PSF2, RING11

Background:

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules.

UniProt Protein Function:	TAP2: a 'transporter associated with antigen processing' (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Transports peptides across the endoplasmic reticulum membrane for assembly of major histocompatibility complex class I molecules. Two subunits, TAP1 and TAP2, are required for peptide transport. ATP hydrolysis is important for transport activity. Nascent MHC class I molecules associate with TAP via tapasin.
UniProt Protein Details:	Protein type:Transporter; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: endoplasmic reticulum membrane; TAP complex; membrane; integral to membrane; integral to endoplasmic reticulum membrane Molecular Function:tapasin binding; protein binding; peptide transporter activity; TAP1 binding; transporter activity; peptide antigen-transporting ATPase activity; ATP binding Biological Process: intracellular transport of viral proteins in host cell; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I; cytosol to ER transport; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; peptide antigen transport; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Disease: Bare Lymphocyte Syndrome, Type I
NCBI Summary:	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]
UniProt Code:	Q03519
NCBI GenInfo Identifier:	549044
NCBI Gene ID:	6891
NCBI Accession:	Q03519.1
UniProt Secondary Accession:	Q03519,O95410, Q53FI6, Q5HY71, Q96PT8, Q9UQ83, B0V2J8
UniProt Related Accession:	Q03519
Molecular Weight:	Calculated: 72kDa/75kDaObserved: 76kDa
NCBI Full Name:	Antigen peptide transporter 2
NCBI Synonym Full Names:	transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
NCBI Official Symbol:	TAP2
NCBI Official Synonym Symbols:	APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
NCBI Protein Information:	antigen peptide transporter 2; ABC transporter, MHC 2; peptide supply factor 2; peptide transporter PSF2; really interesting new gene 11 protein; transporter 2, ABC (ATP binding cassette); peptide transporter involved in antigen processing 2; ATP-binding cassette, sub-family B (MDR/TAP), member 3
UniProt Protein Name:	Antigen peptide transporter 2
UniProt Synonym Protein Names:	ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein
Protein Family:	Protein
UniProt Gene Name:	TAP2
UniProt Entry Name:	TAP2_HUMAN

	Western blot analysis of extracts of various cell lines, using TAP2 antibody (CAB1610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using TAP2 Rabbit pAb (CAB1610) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TAP2 antibody (CAB1610). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)