Anti-TAPBP Antibody (CAB1968)

Antibody Name:	Anti-TAPBP Antibody
Antibody SKU:	CAB1968
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VEDA SGKG LAKR PGAL LLRQ GPGE PPPR PDLD PELY LSVH DPAG ALQA AFRR YPRG APAP HCEM SRFV PLPA SAKW ASGL TPAQ NCPR ALDG AWLM VSIS SPVL SLSS LLRP QPEP QQEP VLIT MATV VLTV LTHT PAPR VRLG QDAL LDLS FAYM PPTS EAAS SLAP GPPP FGLE WRRQ HLGK GHLL LAAT PGLN GQMP AAQE GAVA FAAW DDDE PWGP WTGN GTFW LPT
Gene ID:	6892
Uniprot:	O15533
Cellular Location:	Endoplasmic reticulum membrane, Single-pass type I membrane protein
Calculated MW:	38kDa/43kDa/47kDa/53kDa
Observed MW:	48kDa

Synonyms:

TAPBP, NGS17, TAPA, TPN, TPSN, tapasin

Background:

This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.

UniProt Protein Function:	TAPBP: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; endoplasmic reticulum membrane; TAP complex; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; peptide antigen binding; TAP1 binding; MHC class I protein binding; unfolded protein binding; TAP2 binding; peptide antigen-transporting ATPase activity Biological Process: peptide transport; antigen processing and presentation of peptide antigen via MHC class I; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; peptide antigen stabilization; immune response; protein complex assembly; amide transport; antigen processing and presentation of endogenous peptide antigen via MHC class I Disease: Bare Lymphocyte Syndrome, Type I
NCBI Summary:	This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O15533
NCBI GenInfo Identifier:	12643361
NCBI Gene ID:	6892
NCBI Accession:	O15533.1
UniProt Secondary Accession:	O15533,O15210, O15272, Q5STJ8, Q5STK6, Q5STQ5, A2AB91 A2ABC0, B0V003, B0V0A6, B2ZUA4, E9PGM2,
UniProt Related Accession:	O15533
Molecular Weight:	47,626 Da
NCBI Full Name:	Tapasin
NCBI Synonym Full Names:	TAP binding protein (tapasin)
NCBI Official Symbol:	TAPBP
NCBI Official Synonym Symbols:	TPN; TAPA; TPSN; NGS17
NCBI Protein Information:	tapasin; NGS-17; TAP-binding protein; TAP-associated protein
UniProt Protein Name:	Tapasin
UniProt Synonym Protein Names:	NGS-17; TAP-associated protein; TAP-binding protein
UniProt Gene Name:	TAPBP
UniProt Entry Name:	TPSN_HUMAN

	Western blot analysis of extracts of HT-29 cells, using TAPBP antibody (CAB1968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using TAPBP Antibody (CAB1968) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using TAPBP Antibody (CAB1968) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using TAPBP Antibody (CAB1968) at dilution of 1:200 (40x lens).

Antibodies

Anti-TAPBP Antibody (CAB13357)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)