Anti-TNNC1 Antibody (CAB1927)

Antibody Name:	Anti-TNNC1 Antibody
Antibody SKU:	CAB1927
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TNNC1 (NP_003271.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse heart, Mouse lung, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TNNC1 (NP_003271.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDI YKAA VEQL TEEQ KNEF KAAF DIFV LGAE DGCI STKE LGKV MRML GQNP TPEE LQEM IDEV DEDG SGTV DFDE FLVM MVRC MKDD SKGK SEEE LSDL FRMF DKNA DGYI DLDE LKIM LQAT GETI TEDD IEEL MKDG DKNN DGRI DYDE FLEF MKGV
Gene ID:	7134
Uniprot:	P63316
Cellular Location:
Calculated MW:	18kDa
Observed MW:	16kDa

Synonyms:

TNNC1, CMD1Z, CMH13, TN-C, TNC, TNNC, troponin C1

Background:

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z.

UniProt Protein Function:	TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family.
UniProt Protein Details:	Protein type:Calcium-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: nucleoplasm; troponin complex; mitochondrion; cytosol; actin cytoskeleton Molecular Function:troponin T binding; actin filament binding; protein binding; protein homodimerization activity; troponin I binding; calcium ion binding; calcium-dependent protein binding Biological Process: response to metal ion; regulation of ATPase activity; regulation of muscle contraction; diaphragm contraction; ventricular cardiac muscle morphogenesis; regulation of muscle filament sliding speed; cardiac muscle contraction; muscle filament sliding Disease: Cardiomyopathy, Dilated, 1z; Cardiomyopathy, Familial Hypertrophic, 13
NCBI Summary:	Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]
UniProt Code:	P63316
NCBI GenInfo Identifier:	54042075
NCBI Gene ID:	7134
NCBI Accession:	P63316.1
UniProt Related Accession:	P63316
Molecular Weight:
NCBI Full Name:	Troponin C, slow skeletal and cardiac muscles
NCBI Synonym Full Names:	troponin C1, slow skeletal and cardiac type
NCBI Official Symbol:	TNNC1
NCBI Official Synonym Symbols:	TNC; TN-C; TNNC; CMD1Z; CMH13
NCBI Protein Information:	troponin C, slow skeletal and cardiac muscles
UniProt Protein Name:	Troponin C, slow skeletal and cardiac muscles
Protein Family:	Tenascin
UniProt Gene Name:	TNNC1
UniProt Entry Name:	TNNC1_HUMAN

	Western blot analysis of extracts of various cell lines, using TNNC1 antibody (CAB1927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using TNNC1 antibody (CAB1927) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TNNC1 antibody (CAB1927). Blue: DAPI for nuclear staining.

Antibodies

TNNC1 Antibody (PACO12833)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)