Anti-TPM1 Antibody (CAB1157)

Antibody Name:	Anti-TPM1 Antibody
Antibody SKU:	CAB1157
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat, Zebrafish
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Zebrafish
Positive Samples:	A-549, BT-474, THP-1, DU145, SW480, MCF7, Mouse heart, Mouse skeletal muscle, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGS SSLE AVRR KIRS LQEQ ADAA EERA GTLQ RELD HERK LRET AEAD VASL NRRI QLVE EELD RAQE RLAT ALQK LEEA EKAA DESE RGMK VIES RAQK DEEK MEIQ EIQL KEAK HIAE DADR KYEE VARK LVII ESDL ERAE ERAE LSEG KCAE LEEE LKTV TNNL KSLE AQAE KYSQ KEDR YEEE IKVL SDKL KEAE TRAE FAER SVTK LEKS IDDL EDQL YQQL EQNR RLTN ELKL ALNE D
Gene ID:	7168
Uniprot:	P09493
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	26kDa/28kDa/32kDa
Observed MW:	36kDa

Synonyms:

TPM1, C15orf13, CMD1Y, CMH3, HEL-S-265, HTM-alpha, LVNC9, TMSA

Background:

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.

UniProt Protein Function:	TPM1: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Actin-binding; Motor Chromosomal Location of Human Ortholog: 15q22.1 Cellular Component: filamentous actin; sarcomere; cytoskeleton; stress fiber; cytosol; muscle thin filament tropomyosin Molecular Function:structural constituent of cytoskeleton; structural constituent of muscle; cytoskeletal protein binding; actin binding Biological Process: positive regulation of cell adhesion; wound healing; regulation of muscle contraction; in utero embryonic development; cytoskeleton organization and biogenesis; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; positive regulation of stress fiber formation; ruffle organization and biogenesis; ventricular cardiac muscle morphogenesis; cell motility; negative regulation of cell migration; positive regulation of heart rate by epinephrine; cardiac muscle contraction Disease: Cardiomyopathy, Dilated, 1y; Cardiomyopathy, Familial Hypertrophic, 3
NCBI Summary:	This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Code:	P09493
NCBI GenInfo Identifier:	136092
NCBI Gene ID:	7168
NCBI Accession:	P09493.2
UniProt Secondary Accession:	P09493,P09494, P10469, Q6DV89, Q6DV90, Q7Z6L8, Q86W64 Q96IK2, B7Z5T7, D9YZV2, D9YZV3, D9YZV8,
UniProt Related Accession:	P09493
Molecular Weight:	284
NCBI Full Name:	Tropomyosin alpha-1 chain
NCBI Synonym Full Names:	tropomyosin 1 (alpha)
NCBI Official Symbol:	TPM1
NCBI Official Synonym Symbols:	CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha
NCBI Protein Information:	tropomyosin alpha-1 chain; alpha-tropomyosin; sarcomeric tropomyosin kappa; cardiomyopathy, hypertrophic 3
UniProt Protein Name:	Tropomyosin alpha-1 chain
UniProt Synonym Protein Names:	Alpha-tropomyosin; Tropomyosin-1
Protein Family:	Tropomyosin
UniProt Gene Name:	TPM1
UniProt Entry Name:	TPM1_HUMAN

	Western blot analysis of extracts of various cell lines, using TPM1 antibody (CAB1157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human lung using TPM1 antibody (CAB1157) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using TPM1 antibody (CAB1157) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human uterus using TPM1 antibody (CAB1157) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using TPM1 antibody (CAB1157). Blue: DAPI for nuclear staining.

Antibodies

Anti-Tropomyosin 1 Antibody (CAB8723)

TPM1 Antibody (PACO01656)

TPM1 Antibody (PACO07444)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)