Anti-TXNDC12 Antibody (CAB14403)

Antibody Name:	Anti-TXNDC12 Antibody
Antibody SKU:	CAB14403
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 93-172 of human TXNDC12 (NP_056997.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, 293T, HepG2, Mouse brain, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 93-172 of human TXNDC12 (NP_056997.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLED EEEP KDED FSPD GGYI PRIL FLDP SGKV HPEI INEN GNPS YKYF YVSA EQVV QGMK EAQE RLTG DAFR KKHL EDEL
Gene ID:	51060
Uniprot:	O95881
Cellular Location:	Endoplasmic reticulum lumen
Calculated MW:	19kDa
Observed MW:	19kDa

Synonyms:

TXNDC12, AG1, AGR1, ERP16, ERP18, ERP19, PDIA16, TLP19, hAG-1, hTLP19

Background:

This gene encodes a member of the thioredoxin superfamily. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. This protein localizes to the endoplasmic reticulum and has a single atypical active motif. The encoded protein is mainly involved in catalyzing native disulfide bond formation and displays activity similar to protein-disulfide isomerases. This protein may play a role in defense against endoplasmic reticulum stress. Alternate splicing results in both coding and non-coding variants.

UniProt Protein Function:	TXNDC12: Possesses significant protein thiol-disulfide oxidase activity.
UniProt Protein Details:	Protein type:Secreted; Other Amino Acids Metabolism - glutathione; Oxidoreductase; Secreted, signal peptide; EC 1.8.4.2 Chromosomal Location of Human Ortholog: 1p32.3 Cellular Component: endoplasmic reticulum lumen Molecular Function:peptide disulfide oxidoreductase activity; protein binding
NCBI Summary:	This gene encodes a member of the thioredoxin superfamily. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. This protein localizes to the endoplasmic reticulum and has a single atypical active motif. The encoded protein is mainly involved in catalyzing native disulfide bond formation and displays activity similar to protein-disulfide isomerases. This protein may play a role in defense against endoplasmic reticulum stress. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Mar 2012]
UniProt Code:	O95881
NCBI GenInfo Identifier:	29839456
NCBI Gene ID:	51060
NCBI Accession:	O95881.1
UniProt Secondary Accession:	O95881,Q5T1T4, Q96H50, B3KQS0,
UniProt Related Accession:	O95881
Molecular Weight:	19,206 Da
NCBI Full Name:	Thioredoxin domain-containing protein 12
NCBI Synonym Full Names:	thioredoxin domain containing 12
NCBI Official Symbol:	TXNDC12
NCBI Official Synonym Symbols:	AG1; AGR1; ERP16; ERP18; ERP19; TLP19; hAG-1; PDIA16; hTLP19
NCBI Protein Information:	thioredoxin domain-containing protein 12
UniProt Protein Name:	Thioredoxin domain-containing protein 12
UniProt Synonym Protein Names:	Endoplasmic reticulum resident protein 18; ER protein 18; ERp18; Endoplasmic reticulum resident protein 19; ER protein 19; ERp19; Thioredoxin-like protein p19; hTLP19
Protein Family:	Thioredoxin domain-containing protein
UniProt Gene Name:	TXNDC12
UniProt Entry Name:	TXD12_HUMAN

	Western blot analysis of extracts of various cell lines, using TXNDC12 antibody (CAB14403) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human liver cancer using TXNDC12 Rabbit pAb (CAB14403) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat brain using TXNDC12 Rabbit pAb (CAB14403) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using TXNDC12 Polyclonal Antibody (CAB14403) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using TXNDC12 Polyclonal Antibody (CAB14403) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

TXNDC12 Antibody (PACO12999)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)