Anti-UBE2C Antibody (CAB5499)

Antibody Name:	Anti-UBE2C Antibody
Antibody SKU:	CAB5499
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, THP-1, U-87MG, SW620, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASQ NRDP AATS VAAA RKGA EPSG GAAR GPVG KRLQ QELM TLMM SGDK GISA FPES DNLF KWVG TIHG AAGT VYED LRYK LSLE FPSG YPYN APTV KFLT PCYH PNVD TQGN ICLD ILKE KWSA LYDV RTIL LSIQ SLLG EPNI DSPL NTHA AELW KNPT AFKK YLQE TYSK QVTS QEP
Gene ID:	11065
Uniprot:	O00762
Cellular Location:
Calculated MW:	15kDa/16kDa/17kDa/19kDa
Observed MW:	20kDa

Synonyms:

UBE2C, UBCH10, dJ447F3.2

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.

UniProt Protein Function:	UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:	Protein type:Ligase; Cell cycle regulation; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cyclin catabolic process; exit from mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of exit from mitosis; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of mitotic metaphase/anaphase transition; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process
NCBI Summary:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]
UniProt Code:	O00762
NCBI GenInfo Identifier:	3915178
NCBI Gene ID:	11065
NCBI Accession:	O00762.1
UniProt Secondary Accession:	O00762,A6NP33, E1P5N7, G3XAB7,
UniProt Related Accession:	O00762
Molecular Weight:	17,373 Da
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 C
NCBI Synonym Full Names:	ubiquitin conjugating enzyme E2 C
NCBI Official Symbol:	UBE2C
NCBI Official Synonym Symbols:	UBCH10; dJ447F3.2
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 C
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 C
UniProt Synonym Protein Names:	(E3-independent) E2 ubiquitin-conjugating enzyme C (EC:2.3.2.24
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2C
UniProt Entry Name:	UBE2C_HUMAN

	Western blot analysis of extracts of various cell lines, using UBE2C antibody (CAB5499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using UBE2C antibody (CAB5499) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using UBE2C antibody (CAB5499) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using UBE2C antibody (CAB5499). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug UBE2C antibody (CAB5499). Western blot was performed from the immunoprecipitate using UBE2C antibody (CAB5499) at a dilition of 1:500.

Antibodies	ELISA Kits
Anti-UBE2C Antibody (CAB18075)[KO Validated]	Human UbcH10 / UBE2C ELISA Kit
Anti-UBE2C Antibody (CAB5206)	Human UBE2C (Ubiquitin Conjugating Enzyme E2C) CLIA Kit (HUES00716)
UBE2C Antibody (PACO13022)	Human UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit (HUES02318)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)