The Ube2N/Ubc13 Antibody (CAB10543) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been rigorously tested for use in Western blot applications.UBE2N, also known as Ubc13, is a critical component in the cellular process of adding ubiquitin molecules to protein targets, marking them for degradation or signaling purposes. Dysregulation of the ubiquitin pathway, including UBE2N, has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Ube2N/Ubc13 Antibody
SKU:
CAB10543
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
U-251MG, B cells, Mouse brain, Mouse spleen, Rat brain, Rat testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
17kDa
Observed MW:
17kDa
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Purification Method
Affinity purification
Gene ID
7334
RRID
AB_2758084
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Ube2N/Ubc13 Rabbit pAb (CAB10543) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
