The [KO Validated] UBE2O Antibody (CAB10036) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it reliable for use in Western blot applications. By targeting the UBE2O protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable asset for studies in molecular biology and cancer research.UBE2O, also known as ubiquitin-conjugating enzyme E2O, plays a crucial role in maintaining protein homeostasis by tagging proteins for degradation or regulating their activity through ubiquitination.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] UBE2O Antibody
SKU:
CAB10036
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
E2-230K, 2O
Positive Sample:
A-431, HepG2, Jurkat, Mouse heart, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
141kDa
Observed MW:
200kDa
Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.
Purification Method
Affinity purification
Gene ID
63893
RRID
AB_2757557
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (CAB10036) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from wild type (WT) and UBE2O knockout (KO) HeLa cells, using [KO Validated] UBE2O Rabbit pAb (CAB10036) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunoprecipitation analysis of 200 μg extracts of A-431 cells using 3 μg UBE2O antibody (CAB10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (CAB10036) at a dilution of 1:1000.
