Anti-UBE3A Antibody (CAB1757)

Antibody Name:	Anti-UBE3A Antibody
Antibody SKU:	CAB1757
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human UBE3A (NP_570853.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SKOV3, BT-474, U-251MG, Jurkat, Mouse brain, Mouse heart, Mouse thymus, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human UBE3A (NP_570853.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLIR LWSK YNAD QIRR MMET FQQL ITYK VISN EFNS RNLV NDDD AIVA ASKC LKMV YYAN VVGG EVDT NHNE EDDE EPIP ESSE LTLQ ELLG EERR NKKG PRVD PLET ELGV KTLD CRKP LIPF EEFI NEPL NEVL EMDK DYTF FKVE TENK FSFM TCPF ILNA VTKN LGLY YDNR IRMY SERR ITVL YSLV QGQQ LNPY LRLK VRRD HIID DALV RLEM IAME NPAD LKKQ LYVE FEGE QGVD EGGV SKEF FQLV VEEI FNPD IGMF TYDE STKL FWFN PSSF ETEG QFT
Gene ID:	7337
Uniprot:	Q05086
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	97kDa/100kDa
Observed MW:	110kDa

Synonyms:

UBE3A, ANCR, AS, E6-AP, EPVE6AP, HPVE6A

Background:

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

UniProt Protein Function:	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
NCBI Summary:	This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q05086
NCBI GenInfo Identifier:	215274240
NCBI Gene ID:	7337
NCBI Accession:	Q05086.4
UniProt Secondary Accession:	Q05086,P78355, Q93066, Q9UEP4, Q9UEP5, Q9UEP6, Q9UEP7 Q9UEP8, Q9UEP9, A8K8Z9,
UniProt Related Accession:	Q05086
Molecular Weight:	100,102 Da
NCBI Full Name:	Ubiquitin-protein ligase E3A
NCBI Synonym Full Names:	ubiquitin protein ligase E3A
NCBI Official Symbol:	UBE3A
NCBI Official Synonym Symbols:	AS; ANCR; E6-AP; HPVE6A; EPVE6AP
NCBI Protein Information:	ubiquitin-protein ligase E3A
UniProt Protein Name:	Ubiquitin-protein ligase E3A
UniProt Synonym Protein Names:	E6AP ubiquitin-protein ligase; HECT-type ubiquitin transferase E3A; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54
UniProt Gene Name:	UBE3A

	Western blot analysis of extracts of various cell lines, using UBE3A antibody (CAB1757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using UBE3A Rabbit pAb (CAB1757) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using UBE3A Rabbit pAb (CAB1757) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
UBE3A Antibody (PACO13045)	Human UBE3A ELISA Kit
	Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit (HUES00717)
	Human UBE3A (Ubiquitin Protein Ligase E3A) ELISA Kit (HUES02319)
	Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)