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Anti-UIMC1 Antibody (CAB7244)

SKU:
CAB7244
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-UIMC1 Antibody CAB7244
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-UIMC1 Antibody CAB7244
€139 - €419
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Description

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Antibody Name:Anti-UIMC1 Antibody
Antibody SKU:CAB7244
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UIMC1 (NP_057374.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 293T, Mouse thymus, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UIMC1 (NP_057374.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPRR KKKV KEVS ESRN LEKK DVET TSSV SVKR KRRL EDAF IVIS DSDG EEPK EENG LQKT KTKQ SNRA KCLA KRKI AQMT EEEQ FALA LKMS EQEA REVN SQEE EEEE LLRK AIAE SLNS CRPS DASA TRSR PLAT GPSS QSHQ EKTT DSGL TEGI WQLV PPSL FKGS HISQ GNEA EERE EPWD HTEK TEEE PVSG SSGS WDQS SQPV FENV NVKS FDRC TGHS AEHT QC
Gene ID:51720
Uniprot:Q96RL1
Cellular Location:Nucleus
Calculated MW:17kDa/38kDa/61kDa/70kDa/79kDa
Observed MW:120kDa
Synonyms:UIMC1, RAP80, X2HRIP110
Background:This gene encodes a nuclear protein that interacts with Brca1 (breast cancer 1) in a complex to recognize and repair DNA lesions. This protein binds ubiquitinated lysine 63 of histone H2A and H2AX. This protein may also function as a repressor of transcription. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:RAP80: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Interacts with TSP57. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Belongs to the RAP80 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: nucleus

Molecular Function:retinoid X receptor binding; protein binding; histone binding

Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; double-strand break repair; response to ionizing radiation; negative regulation of transcription, DNA-dependent; G2/M transition DNA damage checkpoint

UniProt Code:Q96RL1
NCBI GenInfo Identifier:60390957
NCBI Gene ID:51720
NCBI Accession:Q96RL1.2
UniProt Secondary Accession:Q96RL1,Q5XKQ1, Q7Z3W7, Q8N5B9, Q9BZR1, Q9BZR5, Q9UHX7 A8MSA1, B3KMZ1, B4E3N2,
UniProt Related Accession:Q96RL1
Molecular Weight:719
NCBI Full Name:BRCA1-A complex subunit RAP80
NCBI Synonym Full Names:ubiquitin interaction motif containing 1
NCBI Official Symbol:UIMC1  
NCBI Official Synonym Symbols:RAP80; X2HRIP110  
NCBI Protein Information:BRCA1-A complex subunit RAP80; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1
UniProt Protein Name:BRCA1-A complex subunit RAP80
UniProt Synonym Protein Names:Receptor-associated protein 80; Retinoid X receptor-interacting protein 110; Ubiquitin interaction motif-containing protein 1
Protein Family:BRCA1-A complex
UniProt Gene Name:UIMC1  
UniProt Entry Name:UIMC1_HUMAN
Anti-UIMC1 Antibody (CAB7244)Western blot analysis of extracts of various cell lines, using UIMC1 antibody (CAB7244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-UIMC1 Antibody (CAB7244)Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using UIMC1 antibody (CAB7244). (Green)GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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