The VPS37A Antibody (CAB14159) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to VPS37A, researchers can accurately detect and analyze its presence in various cell types, making it ideal for investigations in cellular biology and endosomal trafficking.VPS37A is essential for the proper function of the ESCRT machinery, which facilitates the sorting of ubiquitinated cargoes into multivesicular bodies for degradation or recycling.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VPS37A Antibody
SKU:
CAB14159
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCRP1, PQBP2, SPG53, VPS37A
Positive Sample:
MCF7, HepG2, Mouse liver
Cellular Localization:
Late Endosome Membrane, Nucleus, Peripheral Membrane Protein.
Calculated MW:
44kDa
Observed MW:
44kDa
This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
137492
RRID
AB_2761017
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VPS37A Rabbit pAb (CAB14159) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
