Anti-VPS37A Antibody (CAB7853)

Antibody Name:	Anti-VPS37A Antibody
Antibody SKU:	CAB7853
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 224-397 of human VPS37A (NP_689628.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Jurkat, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 224-397 of human VPS37A (NP_689628.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPDV PDAF PELS ELSV SQLT DMNE QEEV LLEQ FLTL PQLK QIIT DKDD LVKS IEEL ARKN LLLE PSLE AKRQ TVLD KYEL LTQM KSTF EKKM QRQH ELSE SCSA SALQ ARLK VAAH EAEE ESDN IAED FLEG KMEI DDFL SSFM EKRT ICHC RRAK EEKL QQAI AMHS QFHA PL
Gene ID:	137492
Uniprot:	Q8NEZ2
Cellular Location:	Late endosome membrane, Nucleus, Peripheral membrane protein
Calculated MW:	20kDa/41kDa/44kDa
Observed MW:	44kDa

Synonyms:

VPS37A, HCRP1, PQBP2, SPG53

Background:

This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	VPS37A: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. Defects in VPS37A are a cause of autosomal recessive complex hereditary spastic paraparesis, a complicated form of hereditary spastic paraplegia (HSP). HSP is a heterogeneous group of neurodegenerative disorders characterized by progressive lower limb spasticity, retrograde degeneration of the crossed cortico- spinal tracts and thinning of the posterior columns in the spinal cord. Complicated forms are characterized by the addition of such neurological features as spastic quadriparesis, seizures, dementia, amyotrophy, extrapyramidal disturbance, cerebral or cerebellar atrophy, optic atrophy, and peripheral neuropathy, as well as by extra neurological manifestations such as dysmorphism, albinism, retinitis pigmentosa, deafness, dementia, amyotrophy and ichthyosis. Individuals with VPS37A mutations present with pronounced early onset spastic paraparesis of upper and lower limbs, mild intellectual disability, kyphosis, pectus carinatum and hypertrichosis. Belongs to the VPS37 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 8p22 Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; late endosome membrane; cytoplasm; endosome membrane Biological Process: protein transport; viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral protein processing; viral infectious cycle; virus assembly; endosome transport Disease: Spastic Paraplegia 53, Autosomal Recessive
NCBI Summary:	This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]
UniProt Code:	Q8NEZ2
NCBI GenInfo Identifier:	74715446
NCBI Gene ID:	137492
NCBI Accession:	Q8NEZ2.1
UniProt Related Accession:	Q8NEZ2
Molecular Weight:	44kDa
NCBI Full Name:	Vacuolar protein sorting-associated protein 37A
NCBI Synonym Full Names:	VPS37A subunit of ESCRT-I
NCBI Official Symbol:	VPS37A
NCBI Official Synonym Symbols:	HCRP1; PQBP2; SPG53
NCBI Protein Information:	vacuolar protein sorting-associated protein 37A
UniProt Protein Name:	Vacuolar protein sorting-associated protein 37A
UniProt Synonym Protein Names:	ESCRT-I complex subunit VPS37A; Hepatocellular carcinoma-related protein 1
Protein Family:	Vacuolar protein sorting-associated protein
UniProt Gene Name:	VPS37A
UniProt Entry Name:	VP37A_HUMAN

	Western blot analysis of extracts of various cell lines, using VPS37A antibody (CAB7853) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using VPS37A antibody (CAB7853) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using VPS37A antibody (CAB7853) at dilution of 1:100 (40x lens).

Antibodies

Anti-VPS37A Antibody (CAB14159)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)