Anti-WHSC1 Antibody (CAB7938)

Antibody Name:	Anti-WHSC1 Antibody
Antibody SKU:	CAB7938
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human WHSC1 (NP_579877.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, HepG2, SW620, Mouse brain, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human WHSC1 (NP_579877.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEFS IKQS PLSV QSVV KCIK MKQA PEIL GSAN GKTP SCEV NREC SVFL SKAQ LSSS LQEG VMQK FNGH DALP FIPA DKLK DLTS RVFN GEPG AHDA KLRF ESQE MKGI GTPP NTTP IKNG SPEI KLKI TKTY MNGK PLFE SSIC GDSA ADVS QSEE NGQK PENK ARRN RKRS IKYD SLLE QGLV EAAL VSKI SSPS DKKI PAKK ESCP NTGR DKDH LLKY NVGD LVWS KVSG YPWW PCMV
Gene ID:	7468
Uniprot:	O96028
Cellular Location:	Chromosome, Cytoplasm, Nucleus
Calculated MW:	30kDa/53kDa/66kDa/69kDa/71kDa/80kDa/152kDa
Observed MW:	80kDa, 152kDa

Synonyms:

NSD2, KMT3F, KMT3G, MMSET, REIIBP, TRX5, WHS, WHSC1

Background:

This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences.

UniProt Protein Function:	WHSC1: a SET domain-containing protein lysine methyltransferase that is expressed normally in early development. Catalyzes the dimethylation of H3K36; H3K36me2 is associated with regions that are transcriptionally active. Maps in the Wolf-Hirschhorn syndrome critical region and is fused to IgH in about 15% of t(4;14) multiple myeloma cases. Patients with the t(4;14) chromosomal translocation comprise one of the worst prognostic subgroups of multiple myeloma. The t(4;14) translocation results in overexpression of WHSC1 and of FGFR3 due to the placement of the strong immunoglobulin H intronic Emu enhancer and 3-prime enhancer in the promoter regions of WHSC1 and FGFR3 genes, respectively. 100% of t(4;14) patients retain overexpression of WHSC1, while 30% have lost expression of FGFR3, suggesting that WHSC1 and not FGFR3 may the primary driver of the disease. Seven alternatively spliced isoforms of the human protein have been reported.
UniProt Protein Details:	Protein type:Oncoprotein; Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine; Ubiquitin conjugating system; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: nucleoplasm; nucleus Molecular Function:histone lysine N-methyltransferase activity (H4-K20 specific); histone-lysine N-methyltransferase activity; protein binding Biological Process: anatomical structure morphogenesis; double-strand break repair via nonhomologous end joining Disease: Wolf-hirschhorn Syndrome
NCBI Summary:	This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq, Jul 2008]
UniProt Code:	O96028
NCBI GenInfo Identifier:	74706096
NCBI Gene ID:	7468
NCBI Accession:	O96028.1
UniProt Secondary Accession:	O96028,O96031, Q4VBY8, Q672J1, Q6IS00, Q86V01, Q9BZB4 A2A2T2, A2A2T3, A2A2T4, A7MCZ1, D3DVQ2,
UniProt Related Accession:	O96028
Molecular Weight:	30,199 Da
NCBI Full Name:	Histone-lysine N-methyltransferase NSD2
NCBI Synonym Full Names:	Wolf-Hirschhorn syndrome candidate 1
NCBI Official Symbol:	WHSC1
NCBI Official Synonym Symbols:	WHS; NSD2; TRX5; MMSET; REIIBP
NCBI Protein Information:	histone-lysine N-methyltransferase NSD2
UniProt Protein Name:	Histone-lysine N-methyltransferase NSD2
UniProt Synonym Protein Names:	Multiple myeloma SET domain-containing protein; MMSET; Nuclear SET domain-containing protein 2; NSD2; Protein trithorax-5; Wolf-Hirschhorn syndrome candidate 1 protein; WHSC1
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	WHSC1
UniProt Entry Name:	NSD2_HUMAN

	Western blot analysis of extracts of various cell lines, using WHSC1 antibody (CAB7938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using WHSC1 antibody (CAB7938) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using WHSC1 antibody (CAB7938) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)