The XCP1 Polyclonal Antibody (CAB22853) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding to the XCP1 protein, this antibody allows for easy detection and analysis in a variety of cell types, making it an ideal tool for studies in cell biology and cancer research.XCP1 plays a crucial role in regulating cell death pathways and maintaining cellular homeostasis. Understanding the function of XCP1 is important in the context of cancer research, as dysregulation of cell death processes can contribute to tumor growth and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Maize samples.
Product Name:
XCP1 Polyclonal Antibody
SKU:
CAB22853
Size:
20μL, 100μL
Reactivity:
Maize
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IDP105, GRMZM2G066326, XCP1, Cysteine protease5
Positive Sample:
B73-329
Cellular Localization:
Vacuole.
Calculated MW:
71kDa
Observed MW:
41kDa
XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis. Results from confocal microscopy and biochemical subcellular fractionation indicated that XCP1 was localized in the vacuole. Ectopic expression of XCP1 resulted in a reduction in plant size in some lines and early leaf senescence, as indicated by early loss of leaf chlorophyll. Reduced plant size was correlated with higher levels of XCP1, as shown by immunoblot and peptidase activity gel analyses. The XCP1 prodomain exhibits exceptionally high similarity (greater than 80%) to the prodomains of papain and other papain-like enzymes isolated from papaya (Carica papaya) laticifers when compared with all other reported papain-like enzymes. The XCP1 and papain to perform common functions as catalysts of autolytic processing following cell death due to programmed suicide or to wounding is discussed.
Purification Method
Affinity purification
Gene ID
100283433
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from B73-329 using XCP1 Rabbit pAb (CAB22853) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 45s.
