Anti-KU70 Antibody (CAB2076)

Antibody Name:	Anti-KU70 Antibody
Antibody SKU:	CAB2076
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human KU70 (NP_001460.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, SKOV3, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human KU70 (NP_001460.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PYFV ALVP QEEE LDDQ KIQV TPPG FQLV FLPF ADDK RKMP FTEK IMAT PEQV GKMK AIVE KLRF TYRS DSFE NPVL QQHF RNLE ALAL DLME PEQA VDLT LPKV EAMN KRLG SLVD EFKE LVYP PDYN PEGK VTKR KHDN EGSG SKRP KVEY SEEE LKTH ISKG TLGK FTVP MLKE ACRA YGLK SGLK KQEL LEAL TKHF QD
Gene ID:	2547
Uniprot:	P12956
Cellular Location:	Chromosome, Nucleus
Calculated MW:	65kDa/69kDa
Observed MW:	68kDa

Synonyms:

XRCC6, CTC75, CTCBF, G22P1, KU70, ML8, TLAA

Background:

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

UniProt Protein Function:	Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
UniProt Protein Details:	Protein type:Helicase; DNA repair, damage; EC 3.6.4.-; Nuclear receptor co-regulator; DNA-binding; RNA-binding; EC 4.2.99.- Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: cytosol; membrane; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleoplasm; nucleus; transcription factor complex Molecular Function:5'-deoxyribose-5-phosphate lyase activity; damaged DNA binding; double-stranded DNA binding; double-stranded telomeric DNA binding; protein binding; protein C-terminus binding Biological Process: DNA ligation; DNA recombination; double-strand break repair via nonhomologous end joining; negative regulation of transcription, DNA-dependent; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein heterotetramerization; regulation of smooth muscle cell proliferation; telomere maintenance
NCBI Summary:	The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
UniProt Code:	P12956
NCBI GenInfo Identifier:	125729
NCBI Gene ID:	2547
NCBI Accession:	P12956.2
UniProt Secondary Accession:	P12956,Q6FG89, Q9UCQ2, Q9UCQ3, B1AHC8,
UniProt Related Accession:	P12956
Molecular Weight:	65,149 Da
NCBI Full Name:	X-ray repair cross-complementing protein 6
NCBI Synonym Full Names:	X-ray repair cross complementing 6
NCBI Official Symbol:	XRCC6
NCBI Official Synonym Symbols:	ML8; KU70; TLAA; CTC75; CTCBF; G22P1
NCBI Protein Information:	X-ray repair cross-complementing protein 6
UniProt Protein Name:	X-ray repair cross-complementing protein 6
UniProt Synonym Protein Names:	5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6
Protein Family:	X-ray repair cross-complementing protein
UniProt Gene Name:	XRCC6
UniProt Entry Name:	XRCC6_HUMAN

	Western blot analysis of extracts of various cell lines, using KU70 antibody (CAB2076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of rat liver, using KU70 antibody (CAB2076) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using KU70 antibody (CAB2076) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-KU70 Antibody (CAB0883)	Ku70/XRCC6 Colorimetric Cell-Based ELISA Kit
Anti-KU70 Antibody (CAB7330)	XRCC6 Colorimetric Cell-Based ELISA
Anti-Ku70 Antibody (CAB11223)	Ku70/XRCC6 (Phospho-Ser5) Colorimetric Cell-Based ELISA Kit
Acetyl-XRCC6 (K539) Antibody (PACO03267)
Acetyl-XRCC6 (K331) Antibody (PACO03268)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)