The ZC3H15 Antibody (CAB10045) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity and cadherin binding activity. Involved in positive regulation of GTPase activity. Located in cytosol.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ZC3H15 Antibody
SKU:
CAB10045
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DFRP1, HT010, LEREPO4, MSTP012, ZC3H15
Positive Sample:
HL-60, SH-SY5Y, LO2, Jurkat, MCF7, Mouse liver
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
49kDa
Observed MW:
65kDa
Enables RNA binding activity and cadherin binding activity. Involved in positive regulation of GTPase activity. Located in cytosol.
Purification Method
Affinity purification
Gene ID
55854
RRID
AB_2757567
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ZC3H15 Rabbit pAb (CAB10045) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.