Anti-ZNF195 Antibody (CAB7347)

Antibody Name:	Anti-ZNF195 Antibody
Antibody SKU:	CAB7347
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ZNF195 (NP_001123991.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, SW620, A-549, Mouse brain, Mouse heart, Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ZNF195 (NP_001123991.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTLL TFRD VAIE FSLE EWKC LDLA QQNL YRDV MLEN YRNL FSVG LTVC KPGL ITCL EQRK EPWN VKRQ EAAD GHPE MGFH HATQ ACLE LLGS SDLP ASAS QSAG ITGV NHRA QPGL NVSV DKFT AMSS HFTQ DLLP EQGI QDAF PKRI LRGY GNCG LDNL YLRK DWES LDEC KLQK DYNG LNQC SSTT HSKI FQYN KYVK IFDN FSNL HRRN ISNT GEKP FKCQ ECGK SFQM LSFL TEHQ KIHT GKKF QK
Gene ID:	7748
Uniprot:	O14628
Cellular Location:	Nucleus
Calculated MW:	10kDa/64kDa/69kDa/70kDa/72kDa
Observed MW:	65kDa

Synonyms:

ZNF195, HRF1, ZNFP104

Background:

This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ZNF195: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: nucleus Molecular Function:DNA binding; zinc ion binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
UniProt Code:	O14628
NCBI GenInfo Identifier:	206729945
NCBI Gene ID:	7748
NCBI Accession:	O14628.2
UniProt Secondary Accession:	O14628,Q0VAJ6, Q658N8, Q6ZNA9, A8K234, B3KTK2, B4DEL0 C9JLY9, L7MNK2,
UniProt Related Accession:	O14628
Molecular Weight:	629
NCBI Full Name:	Zinc finger protein 195
NCBI Synonym Full Names:	zinc finger protein 195
NCBI Official Symbol:	ZNF195
NCBI Official Synonym Symbols:	HRF1; ZNFP104
NCBI Protein Information:	zinc finger protein 195; hypoxia-regulated factor-1; krueppel family zinc finger protein (znfp104)
UniProt Protein Name:	Zinc finger protein 195
Protein Family:	Zinc finger protein
UniProt Gene Name:	ZNF195
UniProt Entry Name:	ZN195_HUMAN

	Western blot analysis of extracts of various cell lines, using ZNF195 antibody (CAB7347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using ZNF195 antibody (CAB7347). Blue: DAPI for nuclear staining.

Antibodies

Anti-ZNF195 Antibody (CAB14071)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)