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ACHE Rabbit Polyclonal Antibody (CAB2806)

SKU:
CAB2806
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Neuroscience
  • Neuroscience Anti-ACHE Antibody CAB2806
  • Neuroscience Anti-ACHE Antibody CAB2806
  • Neuroscience Anti-ACHE Antibody CAB2806
  • Neuroscience Anti-ACHE Antibody CAB2806
  • Neuroscience Anti-ACHE Antibody CAB2806
€139 - €419
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Description

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ACHE Rabbit Polyclonal Antibody (CAB2806)

The Ache Polyclonal Antibody (CAB2806) is a valuable tool for researchers studying the acetylcholinesterase (AChE) enzyme, which plays a crucial role in regulating neurotransmission by breaking down the neurotransmitter acetylcholine. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications.AChE is primarily found in the nervous system and at neuromuscular junctions, where it controls nerve signal transmission. Dysregulation of AChE activity has been linked to various neurological disorders, including Alzheimer's disease and myasthenia gravis. Therefore, studying AChE expression and function is essential for understanding these conditions and developing potential treatments.

With its high reactivity and specificity for human samples, the Ache Polyclonal Antibody (CAB2806) enables accurate detection and analysis of AChE in different cell types and tissues. This makes it an ideal tool for researchers interested in neuroscience, neurology, and drug development focused on targeting the cholinergic system. By investigating AChE expression and activity, valuable insights can be gained into the underlying mechanisms of neurological diseases and potential therapeutic interventions.

Product Name:ACHE Rabbit Polyclonal Antibody
SKU:CAB2806
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 398-617 of human ACHE (NP_056646.1).
Sequence:VPQV SDLA AEAV VLHY TDWL HPED PARL REAL SDVV GDHN VVCP VAQL AGRL AAQG ARVY AYVF EHRA STLS WPLW MGVP HGYE IEFI FGIP LDPS RNYT AEEK IFAQ RLMR YWAN FART GDPN EPRD PKAP QWPP YTAG AQQY VSLD LRPL EVRR GLRA QACA FWNR FLPK LLSA TASE APST CPGF THGE AAPR PGLP LPLL LLHQ LLLL FLSH LRRL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:YT; ACEE; ARACHE; N-ACHE; ACHE
Positive Sample:SH-SY5Y,SGC996,Mouse brain,Mouse spinal cord,Mouse liver,Rat spinal cord
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Nucleus, Peripheral membrane protein, Secreted, synapse.
Calculated MW:68kDa
Observed MW:75kDa

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion

Purification Method:Affinity purification
Gene ID:43
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using ACHE antibody (CAB2806) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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