The BCL2L12 Monoclonal Antibody (CAB19808) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is validated for use in various applications such as Western blot and immunohistochemistry. It specifically binds to the BCL2L12 protein, enabling accurate detection and analysis in human samples.BCL2L12 is a pro-apoptotic protein that plays a role in regulating cell survival and apoptosis. Dysregulation of BCL2L12 has been implicated in various diseases, including cancer.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
BCL2L12 Monoclonal Antibody
SKU:
CAB19808
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2334
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
MCF7, PC-3
Cellular Localization:
Nucleus.
Calculated MW:
37kDa
Observed MW:
32kDa
This gene encodes a member of a family of proteins containing a Bcl-2 homology domain 2 (BH2). The encoded protein is an anti-apoptotic factor that acts as an inhibitor of caspases 3 and 7 in the cytoplasm. In the nucleus, it binds to the p53 tumor suppressor protein, preventing its association with target genes. Overexpression of this gene has been detected in a number of different cancers. There is a pseudogene for this gene on chromosome 3. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
83596
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BCL2L12 Rabbit mAb (CAB19808) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using BCL2L12 Rabbit mAb (CAB19808) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
