The CLOCK Monoclonal Antibody (CAB7265) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, offers high reactivity with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to the CLOCK protein, this antibody allows for accurate detection and analysis in different cell types, making it an ideal tool for studies in chronobiology and related fields. The CLOCK protein plays a crucial role in the control of circadian rhythms and the regulation of physiological processes such as sleep-wake cycles, metabolism, and gene expression. Dysregulation of the CLOCK protein has been linked to disruptions in circadian rhythms, which can contribute to various health conditions, including sleep disorders, metabolic disorders, and mental health issues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CLOCK Monoclonal Antibody
SKU:
CAB7265
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1819
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KAT13D, bHLHe8, CLOCK
Positive Sample:
HeLa, 293T, NIH/3T3, SH-SY5Y, Mouse lung, Rat brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
95kDa
Observed MW:
100kDa
The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
9575
RRID
AB_2863549
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CLOCK Rabbit mAb (CAB7265) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
