The DOK1 Monoclonal Antibody (CAB19250) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 1796 Swiss Prot Synonym pp62; P62DOK; DOK1
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DOK1 Monoclonal Antibody
SKU:
CAB19250
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC2410
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
pp62, P62DOK, DOK1
Positive Sample:
MCF7, BxPC-3
Cellular Localization:
Cytoplasm, Nucleus, Perinuclear Region.
Calculated MW:
52kDa
Observed MW:
62kDa
The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 1796 Swiss Prot Synonym pp62; P62DOK; DOK1
Purification Method:
Affinity purification
Gene ID:
1796
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DOK1 Rabbit mAb (CAB19250) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of BALB-3T3 cells using DOK1 Rabbit mAb (CAB19250) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
