The Stella Antibody (CAB20176) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications such as immunofluorescence and immunohistochemistry.Stella, also known as Dppa3, is a marker for pluripotent stem cells and plays a key role in maintaining the undifferentiated state of these cells. It is involved in regulating gene expression and cell differentiation processes, making it a valuable target for studies in developmental biology and regenerative medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Stella Antibody
SKU:
CAB20176
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCG7, PGC7, Stella, 2410075G02Rik
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Cytoplasm, Female Pronucleus, Male Pronucleus, Nucleus.
Calculated MW:
18kDa
Observed MW:
17kDa
Enables methylated histone binding activity. Involved in negative regulation of DNA demethylation; protection of DNA demethylation of female pronucleus; and regulation of genetic imprinting. Acts upstream of or within embryonic cleavage. Located in cytoplasm; female pronucleus; and male pronucleus. Is expressed in several structures, including central nervous system; early conceptus; gonad; hindgut diverticulum; and primordial germ cell. Orthologous to human DPPA3 (developmental pluripotency associated 3).
Purification Method
Affinity purification
Gene ID
73708
RRID
AB_2862963
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Stella Rabbit pAb (CAB20176) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
