The ATP1A4 Antibody (CAB15256) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, demonstrates high specificity and reactivity towards human samples, making it an ideal choice for Western blot applications.ATP1A4, a member of the ATPase family, is responsible for maintaining the electrochemical gradient across cell membranes by pumping sodium and potassium ions in and out of cells. Dysregulation of ATP1A4 activity has been linked to various diseases, including neurological disorders and cardiovascular conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ATP1A4 Antibody
SKU:
CAB15256
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATP1A1, ATP1AL2, ATP1A4
Positive Sample:
Mouse testis, Mouse brain, Mouse kidney, Rat testis, Rat brain, Rat kidney
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
114kDa
Observed MW:
100kDa
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
480
RRID
AB_2762154
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP1A4 Rabbit pAb (CAB15256) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1S.
