Anti-ATP5J2 Antibody (CAB17055)

Antibody Name:	Anti-ATP5J2 Antibody
Antibody SKU:	CAB17055
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human ATP5J2.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:

Immunogen:	A synthetic peptide of human ATP5J2.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	9551
Uniprot:	P56134
Cellular Location:
Calculated MW:
Observed MW:	Refer to figures

Synonyms:

ATP5J2, ATP5JL

Background:

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene.

UniProt Protein Function:	ATP5J2: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase F chain family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Energy Metabolism - oxidative phosphorylation; Hydrolase; Mitochondrial Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: proton-transporting ATP synthase complex, coupling factor F(o); mitochondrial inner membrane; integral to membrane; nucleus; mitochondrial proton-transporting ATP synthase complex Molecular Function:ATPase activity; transmembrane transporter activity Biological Process: cellular metabolic process; proton transport; mitochondrial ATP synthesis coupled proton transport
NCBI Summary:	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene. [provided by RefSeq, Nov 2010]
UniProt Code:	P56134
NCBI GenInfo Identifier:	51479129
NCBI Gene ID:	9551
NCBI Accession:	NP_001003713.1
UniProt Secondary Accession:	P56134,O76079, Q6IBB3, Q96L83, Q9BTI8, C9J8H9, F8W7V3
UniProt Related Accession:	P56134
Molecular Weight:
NCBI Full Name:	ATP synthase subunit f, mitochondrial isoform 2b
NCBI Synonym Full Names:	ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2
NCBI Official Symbol:	ATP5J2
NCBI Official Synonym Symbols:	ATP5JL
NCBI Protein Information:	ATP synthase subunit f, mitochondrial
UniProt Protein Name:	ATP synthase subunit f, mitochondrial
UniProt Gene Name:	ATP5J2
UniProt Entry Name:	ATPK_HUMAN

Western blot analysis of extracts of various cell lines, using ATP5J2 antibody (CAB17055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)