The BIN2 Polyclonal Antibody (CAB21175) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit immunoglobulins, exhibits high specificity and sensitivity when used in Western blot applications, allowing for accurate detection and analysis of BIN2 protein levels in various cell types.BIN2, also known as brassinosteroid insensitive 2, is involved in plant growth and development, particularly in the regulation of brassinosteroid signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
BIN2 Polyclonal Antibody
SKU:
CAB21175
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Encodes BIN2, a member of the ATSK (shaggy-like kinase) family. BIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 regulates root epidermal cell fate specification by phosphorylating EGL3 and TTG1. BIN2-mediated phosphorylation appears to promote BZR1 export from the nucleus.
Purification Method
Affinity purification
Gene ID
827605
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit pAb (CAB21175) at 1:200 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
