Description
Bovine Chemokine Array (9 targets) (SARB0001)
The Bovine Chemokine Array 9 Targets (SARB0001) from Assay Genie is a powerful tool for researchers studying chemokine signaling in bovine samples. This array kit allows for the simultaneous detection and quantification of 9 different chemokine targets, providing insight into the inflammatory response and immune regulation in bovine systems.With a high level of sensitivity and specificity, the Bovine Chemokine Array 9 Targets offers researchers the ability to analyze multiple targets in a single experiment, saving time and resources.
The array is easy to use and provides reliable and reproducible results, making it an essential tool for studies in immunology, inflammation, and infectious diseases in bovine models.Whether investigating immune responses to infection, studying inflammatory pathways in disease states, or exploring the role of chemokines in bovine health and wellness, the Bovine Chemokine Array 9 Targets from Assay Genie is a valuable resource for researchers seeking to unravel the complexities of chemokine signaling in bovine biology.
| Product Sku: | SARB0001 |
| Size: | 2, 4, or 8 |
| Species Detected: | Bovine |
| Number of Targets Detected: | 9 |
| Gene Symbols: | CCL2, CXCL8, CXCL9, IFNG, IL1A, IL1B, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
MIG (CXCL9)
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-8 (CXCL8)
|
MCP-1 (CCL2)
| MIP-1 beta (CCL4)
| RANTES (CCL5) | TNF alpha
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Bovine Chemokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
