Description
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Bovine Cytokine Array (30 targets) (SARB0002)
The Bovine Cytokine Array (SARB0002) is a comprehensive tool for researchers studying cytokine expression in bovine samples. This array allows for simultaneous detection and quantification of 30 different cytokines, providing valuable insights into the immune response and inflammatory processes in bovines.With its high sensitivity and specificity, this cytokine array offers a reliable and efficient way to analyze cytokine profiles in various sample types, including serum, plasma, and cell culture supernatants. Researchers can easily identify changes in cytokine levels and explore potential biomarkers for disease diagnosis and monitoring.
This versatile tool is suitable for a wide range of research applications, including immunology, veterinary medicine, and agriculture. By studying cytokine expression patterns in bovines, researchers can gain a deeper understanding of immune regulation and develop innovative strategies for disease prevention and treatment. Unlock the potential of bovine cytokine research with the Bovine Cytokine Array (SARB0002).
| Product Sku: | SARB0002 |
| Size: | 2, 4, or 8 |
| Species Detected: | Bovine |
| Number of Targets Detected: | 30 |
| Gene Symbols: | ANGPT1, C1QTNF1, CCL2, CD40LG, CXCL10, CXCL9, DCN, FGF1, FGF2, IFNG|IGF1, IL10, IL13, IL15, IL17A, IL18, IL1A, IL1B, IL2, IL21, IL36RN, IL4, LIF, NCAM1, VEGFA |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
aFGF (FGF-1)
| Angiopoietin-1
| bFGF
| CD40 Ligand (TNFSF5)
| Decorin
|
GASP-1 (WFIKKNRP)
| IFN alpha
| IFN-beta
| IFN-gamma
| IGF-1
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-36 Ra (IL-1 F5)
| IL-10
| IL-13
|
IL-15
| IL-17A
| IL-18
| IL-2
| IL-21
|
IL-4
| IP-10 (CXCL10)
| LIF
| MCP-1 (CCL2)
| MIG (CXCL9)
|
MIP-1 beta (CCL4)
| NCAM-1 (CD56)
| RANTES (CCL5) | TNF alpha
| VEGF-A
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Bovine Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
