Anti-BRD7 Antibody (CAB2308)

Antibody Name:	Anti-BRD7 Antibody [KO Validated]
Antibody SKU:	CAB2308
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human BRD7 (NP_037395.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, HepG2, SW620, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human BRD7 (NP_037395.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKK HKKH KSDK HLYE EYVE KPLK LVLK VGGN EVTE LSTG SSGH DSSL FEDK NDHD KHKD RKRK KRKK GEKQ IPGE EKGR KRRR VKED KKKR DRD
Gene ID:	29117
Uniprot:	Q9NPI1
Cellular Location:	Nucleus
Calculated MW:	74kDa
Observed MW:	100kDa

Synonyms:

BRD7, BP75, CELTIX1, NAG4

Background:

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	BRD7: a bromodomain protein that binds dishevelled-1 (DVL1) and enhances Wnt signaling by inhibiting GSK3B. Interacts with DVL1, PTPN13, IRF2 and HNRPUL1. Associates with histones and E1B-AP5. Binds acetylated histone peptides, most notably H3 peptide acetylated at Lys14. Associates with histones and E1B-AP5. Its bromodomain is found in many chromatin-associated proteins and most known histone acetyltransferases. Inhibits G1-S progression by transcriptional regulation of molecules in the ras and Rb pathways. Plays an important role in transcriptional activation and chromatin remodeling. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription regulation; Tumor suppressor; Cell cycle regulation; Histone-binding Chromosomal Location of Human Ortholog: 16q12 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; histone binding; p53 binding; transcription coactivator activity; transcription factor binding; transcription corepressor activity Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of histone acetylation; negative regulation of transcription, DNA-dependent; cell cycle
NCBI Summary:	This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
UniProt Code:	Q9NPI1
NCBI GenInfo Identifier:	74734307
NCBI Gene ID:	29117
NCBI Accession:	Q9NPI1.1
UniProt Secondary Accession:	Q9NPI1,Q4VC09, Q8N2L9, Q96KA4, Q9BV48, Q9UH59,
UniProt Related Accession:	Q9NPI1
Molecular Weight:	651
NCBI Full Name:	Bromodomain-containing protein 7
NCBI Synonym Full Names:	bromodomain containing 7
NCBI Official Symbol:	BRD7
NCBI Official Synonym Symbols:	BP75; NAG4; CELTIX1
NCBI Protein Information:	bromodomain-containing protein 7; protein CELTIX-1; 75 kDa bromodomain protein
UniProt Protein Name:	Bromodomain-containing protein 7
UniProt Synonym Protein Names:	75 kDa bromodomain protein; Protein CELTIX-1
Protein Family:	Bromodomain-containing protein
UniProt Gene Name:	BRD7
UniProt Entry Name:	BRD7_HUMAN

	Western blot analysis of extracts of various cell lines, using BRD7 antibody (CAB2308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and BRD7 Rabbit pAb knockout (KO) 293T cells, using BRD7 Rabbit pAb antibody (CAB2308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.

Antibodies

BRD7 Antibody (PACO08043)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)