Anti-Alpha-Catenin Antibody (CAB5635)

Antibody Name:	Anti-Alpha-Catenin Antibody [KO Validated]
Antibody SKU:	CAB5635
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human Alpha-Catenin (NP_001894.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, OVCAR3, 293T, MCF7, Mouse lung, Mouse heart, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human Alpha-Catenin (NP_001894.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ELDD SDFE TEDF DVRS RTSV QTED DQLI AGQS ARAI MAQL PQEQ KAKI AEQV ASFQ EEKS KLDA EVSK WDDS GNDI IVLA KQMC MIMM EMTD FTRG KGPL KNTS DVIS AAKK IAEA GSRM DKLG RTIA DHCP DSAC KQDL LAYL QRIA LYCH QLNI CSKV KAEV QNLG GELV VSGV DSAM SLIQ AAKN LMNA VVQT VKAS YVAS TKYQ KSQG MASL NLPA VSWK MKAP EKKP LVKR EKQD ETQT KIKR ASQK KHVN PVQA LSEF KAMD SI
Gene ID:	1495
Uniprot:	P35221
Cellular Location:	Cell junction, Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton
Calculated MW:	59kDa/100kDa/102kDa
Observed MW:	115kDa

Synonyms:

CAP102, MDPT2, Catenin alpha 1, CTNNA1

Background:

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy.

UniProt Protein Function:	CTNNA1: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F- actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Expressed ubiquitously in normal tissues. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 5q31.2 Cellular Component: focal adhesion; lamellipodium; acrosome; plasma membrane; catenin complex; zonula adherens; intercellular junction; cytosol; actin cytoskeleton Molecular Function:actin filament binding; protein binding; cadherin binding; gamma-catenin binding; beta-catenin binding; structural molecule activity; vinculin binding Biological Process: intercellular junction assembly and maintenance; apical junction assembly; gap junction assembly; protein heterooligomerization; axon regeneration; male gonad development; establishment and/or maintenance of cell polarity; actin filament organization; odontogenesis of dentine-containing teeth; muscle cell differentiation; negative regulation of neuroblast proliferation; ovarian follicle development; response to estrogen stimulus; positive regulation of smoothened signaling pathway; positive regulation of muscle cell differentiation; cell adhesion; vascular endothelial growth factor receptor signaling pathway; aging
UniProt Code:	P35221
NCBI GenInfo Identifier:	461853
NCBI Gene ID:	1495
NCBI Accession:	P35221.1
UniProt Secondary Accession:	P35221,Q12795, Q8N1C0,
UniProt Related Accession:	P35221
Molecular Weight:	59,550 Da
NCBI Full Name:	Catenin alpha-1
NCBI Synonym Full Names:	catenin (cadherin-associated protein), alpha 1, 102kDa
NCBI Official Symbol:	CTNNA1
NCBI Official Synonym Symbols:	CAP102
NCBI Protein Information:	catenin alpha-1; alpha-catenin; alphaE-catenin; alpha E-catenin; alpha-E-catenin; renal carcinoma antigen NY-REN-13; cadherin-associated protein,102kDa
UniProt Protein Name:	Catenin alpha-1
UniProt Synonym Protein Names:	Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13
Protein Family:	Catenin
UniProt Gene Name:	CTNNA1
UniProt Entry Name:	CTNA1_HUMAN

	Western blot analysis of extracts of various cell lines, using alpha-Catenin antibody (CAB5635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and alpha-Catenin Rabbit pAb knockout (KO) 293T cells, using a-Catenin Rabbit pAb antibody (CAB5635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat kidney using Alpha-Catenin antibody (CAB5635) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human vermiform appendix using Alpha-Catenin antibody (CAB5635) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using Alpha-Catenin antibody (CAB5635) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Alpha-Catenin Antibody [KO Validated] (CAB19004)	Human CTNN alpha1 (Catenin, Alpha 1) ELISA Kit (HUES01823)
Phospho-CTNNA1 (S641) Antibody (PACO03534)	Catenin-alpha1 Colorimetric Cell-Based ELISA Kit
CTNNA1 Antibody (PACO03535)
CTNNA1 Antibody (PACO08658)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)