Anti-CBX5 Antibody (CAB12577)

Antibody Name:	Anti-CBX5 Antibody [KO Validated]
Antibody SKU:	CAB12577
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CBX5 (NP_001120794.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Jurkat, SH-SY5Y, A-549, MCF7, Mouse brain, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CBX5 (NP_001120794.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKK TKRT ADSS SSED EEEY VVEK VLDR RVVK GQVE YLLK WKGF SEEH NTWE PEKN LDCP ELIS EFMK KYKK MKEG ENNK PREK SESN KRKS NFSN SADD
Gene ID:	23468
Uniprot:	P45973
Cellular Location:	Chromosome, Nucleus, centromere
Calculated MW:	22kDa
Observed MW:	22kDa

Synonyms:

CBX5, HEL25, HP1, HP1A

Background:

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: kinetochore; nucleoplasm; PML body; histone methyltransferase complex; transcriptional repressor complex; histone deacetylase complex; nucleolus; nuclear envelope; nuclear heterochromatin; nucleus; chromocenter Molecular Function:protein binding, bridging; protein binding; protein homodimerization activity; histone deacetylase binding; chromatin binding; methylated histone residue binding Biological Process: viral reproduction; blood coagulation; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P45973
NCBI GenInfo Identifier:	1170338
NCBI Gene ID:	23468
NCBI Accession:	P45973.1
UniProt Secondary Accession:	P45973,B2R8T9,
UniProt Related Accession:	P45973
Molecular Weight:	191
NCBI Full Name:	Chromobox protein homolog 5
NCBI Synonym Full Names:	chromobox homolog 5
NCBI Official Symbol:	CBX5
NCBI Official Synonym Symbols:	HP1; HP1A; HEL25
NCBI Protein Information:	chromobox protein homolog 5; HP1-ALPHA; HP1Hs alpha; antigen p25; HP1 alpha homolog; epididymis luminal protein 25; heterochromatin protein 1-alpha; heterochromatin protein 1 homolog alpha; chromobox homolog 5 (HP1 alpha homolog, Drosophila)
UniProt Protein Name:	Chromobox protein homolog 5
UniProt Synonym Protein Names:	Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha
Protein Family:	Chromobox protein
UniProt Gene Name:	CBX5
UniProt Entry Name:	CBX5_HUMAN

	Western blot analysis of extracts of various cell lines, using CBX5 antibody (CAB12577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and CBX5 Rabbit pAb knockout (KO) 293T cells, using CBX5 Rabbit pAb antibody (CAB12577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse lung using CBX5 antibody (CAB12577) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using CBX5 antibody (CAB12577) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CBX5 Antibody (CAB1098)[KO Validated]	CBX5 Colorimetric Cell-Based ELISA
Anti-CBX5 Antibody (CAB12592)
Anti-HP1 alpha Antibody (CAB3741)
CBX5 Antibody (PACO00934)
Phospho-CBX5 (S92) Antibody (PACO03704)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)