Anti-CD86 Antibody (CAB1199)

Antibody Name:	Anti-CD86 Antibody
Antibody SKU:	CAB1199
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, Mouse thymus, Mouse spleen, Mouse kidney, Rat spleen, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AYFN ETAD LPCQ FANS QNQS LSEL VVFW QDQE NLVL NEVY LGKE KFDS VHSK YMGR TSFD SDSW TLRL HNLQ IKDK GLYQ CIIH HKKP TGMI RIHQ MNSE LSVL ANFS QPEI VPIS NITE NVYI NLTC SSIH GYPE PKKM SVLL RTKN STIE YDGI MQKS QDNV TELY DVSI SLSV SFPD VTSN MTIF CILE TDKT RLLS SPFS IELE DPQP PPDH IP
Gene ID:	942
Uniprot:	P42081
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	12kDa/24kDa/28kDa/31kDa/37kDa
Observed MW:	68kDa/80kDa

Synonyms:

B7-2, B7.2, B70, CD28LG2, LAB72, CD86

Background:

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

UniProt Protein Function:	CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Immunoglobulin superfamily; Membrane protein, integral Chromosomal Location of Human Ortholog: 3q21 Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane Molecular Function:protein binding; coreceptor activity; receptor activity; receptor binding Biological Process: positive regulation of lymphotoxin A biosynthetic process; negative regulation of T cell anergy; T cell activation; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; response to yeast; defense response to virus; aging; response to drug; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response
NCBI Summary:	This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
UniProt Code:	P42081
NCBI GenInfo Identifier:	317373339
NCBI Gene ID:	942
NCBI Accession:	P42081.2
UniProt Secondary Accession:	P42081,Q13655, Q6FHB1, Q6GTS4, Q7M4L5, A0N0P0, B7Z2F3 B7Z702, E7ETN5, E9PC27,
UniProt Related Accession:	P42081
Molecular Weight:	329
NCBI Full Name:	T-lymphocyte activation antigen CD86
NCBI Synonym Full Names:	CD86 molecule
NCBI Official Symbol:	CD86
NCBI Official Synonym Symbols:	B70; B7-2; B7.2; LAB72; CD28LG2
NCBI Protein Information:	T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
UniProt Protein Name:	T-lymphocyte activation antigen CD86
UniProt Synonym Protein Names:	Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD_antigen: CD86
UniProt Gene Name:	CD86
UniProt Entry Name:	CD86_HUMAN

	Western blot analysis of extracts of THP-1 cells, using CD86 Antibody (CAB1199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of various cell lines, using CD86 antibody (CAB1199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
	Immunohistochemistry of paraffin-embedded mouse spleen using CD86 Rabbit pAb (CAB1199) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Raw264.7 cells using CD86 antibody (CAB1199) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Human CD86 Monoclonal Antibody [BU63](AGEL0092)	Human CD86 / B7-2 ELISA Kit
Human CD86 Monoclonal Antibody (Biotin Conjugated) [BU63](AGEL0093)	Human LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2) CLIA Kit (HUES00924)
Human CD86 Monoclonal Antibody (FITC Conjugated) [BU63](AGEL0094)	Human T-lymphocyte activation antigen CD86 (CD86) ELISA Kit
Human CD86 Monoclonal Antibody (Alexa Fluor 488 Conjugated) [BU63](AGEL0095)
Human CD86 Monoclonal Antibody (PE Conjugated) [BU63](AGEL0644)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)