Anti-CLNS1A Antibody (CAB16040)

Antibody Name:	Anti-CLNS1A Antibody
Antibody SKU:	CAB16040
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-237 of human CLNS1A (NP_001284.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, 293T, DU145, Jurkat, HeLa, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-237 of human CLNS1A (NP_001284.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSFL KSFP PPGP AEGL LRQQ PDTE AVLN GKGL GTGT LYIA ESRL SWLD GSGL GFSL EYPT ISLH ALSR DRSD CLGE HLYV MVNA KFEE ESKE PVAD EEEE DSDD DVEP ITEF RFVP SDKS ALEA MFTA MCEC QALH PDPE DEDS DDYD GEEY DVEA HEQG QGDI PTFY TYEE GLSH LTAE GQAT LERL EGML SQSV SSQY NMAG VRTE DSIR DYED GMEV DTTP TVAG QFED ADVD H
Gene ID:	1207
Uniprot:	P54105
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, cytosol
Calculated MW:	26kDa
Observed MW:	38kDa

Synonyms:

CLNS1A, CLCI, CLNS1B, ICln

Background:

This gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Pseudogenes of this gene are found on chromosomes 1, 4 and 6. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ICLN: The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. Belongs to the pICln (TC 1.A.47) family.
UniProt Protein Details:	Protein type:Channel, chloride Chromosomal Location of Human Ortholog: 11q13.5-q14 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:protein binding; protein heterodimerization activity Biological Process: spliceosomal snRNP biogenesis
NCBI Summary:	This gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Pseudogenes of this gene are found on chromosomes 1, 4 and 6. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
UniProt Code:	P54105
NCBI GenInfo Identifier:	1708393
NCBI Gene ID:	1207
NCBI Accession:	P54105.1
UniProt Secondary Accession:	P54105,Q0VDK6, Q9NRD2, Q9NRD3, B2RCS9,
UniProt Related Accession:	P54105
Molecular Weight:	26,215 Da
NCBI Full Name:	Methylosome subunit pICln
NCBI Synonym Full Names:	chloride nucleotide-sensitive channel 1A
NCBI Official Symbol:	CLNS1A
NCBI Official Synonym Symbols:	CLCI; ICln; CLNS1B
NCBI Protein Information:	methylosome subunit pICln
UniProt Protein Name:	Methylosome subunit pICln
UniProt Synonym Protein Names:	Chloride channel, nucleotide sensitive 1A; Chloride conductance regulatory protein ICln; I(Cln); Chloride ion current inducer protein; ClCI; Reticulocyte pICln
UniProt Gene Name:	CLNS1A
UniProt Entry Name:	ICLN_HUMAN

	Western blot analysis of extracts of various cell lines, using CLNS1A antibody (CAB16040) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat kidney using CLNS1A antibody (CAB16040) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using CLNS1A antibody (CAB16040) at dilution of 1:100 (40x lens).

Antibodies

Anti-CLNS1A Antibody (CAB9739)

CLNS1A Antibody (PACO01337)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)