The DAP Kinase 1 (DAPK1) Antibody (CAB5741) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to DAPK1, this antibody enables the detection and analysis of this important protein in a variety of cell types.DAPK1, also known as Death-associated protein kinase 1, is involved in a variety of cellular processes, including apoptosis, autophagy, and immune response regulation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DAP Kinase 1 (DAPK1) Antibody
SKU:
CAB5741
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAPK, ROCO3, DAP Kinase 1 (DAPK1)
Positive Sample:
Mouse lung, Mouse kidney
Cellular Localization:
Cytoplasm, Cytoskeleton, Cytoskeleton.
Calculated MW:
160kDa
Observed MW:
160kDa
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1612
RRID
AB_2766497
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DAP Kinase 1 (DAPK1) Rabbit pAb (CAB5741) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
