The Dimethyl-Histone H3 (K79) Polyclonal Antibody (CAB2368) offered by AssayGenie is a high-quality antibody designed for research involving histone methylation, specifically targeting histone H3 at lysine 79 (K79) site. This antibody, raised in rabbits, is highly reactive with samples from various species and has been validated for use in a range of applications including Western blot, immunohistochemistry, and chromatin immunoprecipitation (ChIP). It specifically binds to dimethylated histone H3 at lysine 79, allowing for precise detection and analysis of this histone modification in different cell types and experimental conditions.Histone methylation at lysine 79 has been implicated in various biological processes including gene regulation, DNA repair, and chromatin structure.
Dysregulation of this modification has been associated with diseases such as cancer, neurodevelopmental disorders, and autoimmune conditions. By using this antibody, researchers can gain a deeper understanding of the role of histone H3 lysine 79 methylation in these disease pathways, potentially leading to the development of novel therapeutic strategies.Overall, the Dimethyl-Histone H3 (K79) Polyclonal Antibody (CAB2368) from AssayGenie is a valuable tool for scientists and researchers studying epigenetic modifications and their impact on cellular processes and disease mechanisms.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and DiMethyl-Histone H3-K79 Rabbit pAb (CAB2368). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH), as shown in figure.
