The EGFR Polyclonal Antibody (CAB11352) is a versatile tool for researchers studying the epidermal growth factor receptor (EGFR), a key protein involved in cell growth, proliferation, and survival. This polyclonal antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications.EGFR is a crucial signaling molecule implicated in various cancers, making this antibody ideal for studies in cancer research and targeted therapy development. By binding to EGFR, this antibody enables precise detection and analysis of the protein in different cell types, providing valuable insights into its role in cancer progression and potential therapeutic strategies.
With its ability to target EGFR, the antibody opens up opportunities for investigating the mechanisms underlying tumor growth and sensitivity to treatment, ultimately contributing to the development of personalized medicine approaches for cancer patients. Researchers exploring EGFR signaling pathways and potential therapeutic interventions will find this antibody to be a valuable asset in their studies.
Product Name:
EGFR Rabbit Polyclonal Antibody
SKU:
CAB11352
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using EGFR Rabbit pAb (CAB11352) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
