Anti-EIF4E Antibody (CAB2162)

Antibody Name:	Anti-EIF4E Antibody
Antibody SKU:	CAB2162
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, SW620, MCF-7, 293T, Mouse brain, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATV EPET TPTP NPPT TEEE KTES NQEV ANPE HYIK HPLQ NRWA LWFF KNDK SKTW QANL RLIS KFDT VEDF WALY NHIQ LSSN LMPG CDYS LFKD GIEP
Gene ID:	1977
Uniprot:	P06730
Cellular Location:	Cytoplasm, P-body
Calculated MW:	25kDa/27kDa/28kDa
Observed MW:	25kDa

Synonyms:

AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E, EIF4E

Background:

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.
UniProt Protein Details:	Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 4q23 Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol Molecular Function:protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; G1/S transition of mitotic cell cycle; lung development Disease: Autism, Susceptibility To, 19
NCBI Summary:	The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:	P06730
NCBI GenInfo Identifier:	1352435
NCBI Gene ID:	1977
NCBI Accession:	P06730.2
UniProt Secondary Accession:	P06730,Q96E95, B7Z6V1, D6RCQ6,
UniProt Related Accession:	P06730
Molecular Weight:	27,260 Da
NCBI Full Name:	Eukaryotic translation initiation factor 4E
NCBI Synonym Full Names:	eukaryotic translation initiation factor 4E
NCBI Official Symbol:	EIF4E
NCBI Official Synonym Symbols:	CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
NCBI Protein Information:	eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
UniProt Protein Name:	Eukaryotic translation initiation factor 4E
UniProt Synonym Protein Names:	eIF-4F 25 kDa subunit; mRNA cap-binding protein
Protein Family:	Eukaryotic translation initiation factor
UniProt Gene Name:	EIF4E
UniProt Entry Name:	IF4E_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF4E antibody (CAB2162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody (CAB2162) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody (CAB2162) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using EIF4E antibody (CAB2162) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EIF4E antibody (CAB2162). Western blot was performed from the immunoprecipitate using EIF4E antibody (CAB2162) at a dilition of 1:1000.

Antibodies

Anti-EIF4E Antibody (CAB0468)

Anti-EIF4E Antibody (CAB6085)

Anti-Phospho-eIF4E-S209 Antibody (CABP0747)

Anti-EIF4E Antibody (CAB19044)

Phospho-EIF4E (S209) Antibody (PACO00205)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)