eNOS Rabbit Polyclonal Antibody (CAB15075)

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CAB15075
  • Western blot analysis of extracts of various cell lines, using eNOS antibody (CAB15075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
  • Western blot analysis of extracts of EV304 cells, using eNOS antibody (CAB15075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
  • Immunofluorescence analysis of HUVEC cells using NOS3 antibody (CAB15075) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
€99 - €249

Description

Technical Manual

eNOS Rabbit Polyclonal Antibody

Product Name:eNOS Rabbit Polyclonal Antibody
Product SKU:CAB15075
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 680-780 of human eNOS (NP_000594.2).
Sequence:

LQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATIL

Gene ID:4846
Swiss Prot:P29474
Synonyms:NOS3; ECNOS; eNOS
Calculated MW:67kDa/68kDa/133kDa
Observed MW:150KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:293T, EV304, Mouse liver
Cellular Location:Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton

Product Images

eNOS Rabbit Polyclonal Antibody (CAB15075)

Western blot analysis of extracts of various cell lines, using eNOS antibody (CAB15075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.

eNOS Rabbit Polyclonal Antibody (CAB15075)

Western blot analysis of extracts of EV304 cells, using eNOS antibody (CAB15075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.

eNOS Rabbit Polyclonal Antibody (CAB15075)

Immunofluorescence analysis of HUVEC cells using NOS3 antibody (CAB15075) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function:actin monomer binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding

Biological Process: angiogenesis; arginine catabolic process; blood coagulation; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; nitric oxide metabolic process; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vascular endothelial growth factor receptor signaling pathway

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic

NCBI Summary:Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Code:P29474
NCBI GenInfo Identifier:266648
NCBI Gene ID:4846
NCBI Accession:P29474.3
UniProt Secondary Accession:P29474,Q13662, Q14251, Q14434, Q548C1, Q6GSL5, Q9UDC6 A0S0A7, A0S0A8, A8KA63, B2RCQ1, E9PFR2,
UniProt Related Accession:P29474
Molecular Weight:
NCBI Full Name:Nitric oxide synthase, endothelial
NCBI Synonym Full Names:nitric oxide synthase 3
NCBI Official Symbol:NOS3 
NCBI Official Synonym Symbols:eNOS; ECNOS 
NCBI Protein Information:nitric oxide synthase, endothelial
UniProt Protein Name:Nitric oxide synthase, endothelial
UniProt Synonym Protein Names:Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Protein Family:Nitric oxide synthase
UniProt Gene Name:NOS3 
UniProt Entry Name:NOS3_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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