The Phospho-eNOS-S1177 Monoclonal Antibody (CABP1404) is a high-quality antibody developed for reliable detection and analysis of target proteins. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. RRID Gene ID 4846 Swiss Prot Synonym eNOS; ECNOS; Phospho-eNOS-S1177
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-eNOS-S1177 Monoclonal Antibody
SKU:
CABP1404
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC58017
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:8000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. RRID Gene ID 4846 Swiss Prot Synonym eNOS; ECNOS; Phospho-eNOS-S1177
Purification Method:
Affinity purification
Gene ID:
4846
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HUVEC cells using Phospho-eNOS-S1177 Rabbit mAb (CABP1404) at 1:6000 dilution incubated overnight at 4℃. HUVEC cells were treated with Pervanadate (100 μM) at 37℃ for 10 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
