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Anti-EPHA4 Antibody (CAB8346)

SKU:
CAB8346
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-EPHA4 Antibody CAB8346
  • Cell Biology Antibodies 12 Anti-EPHA4 Antibody CAB8346
€139 - €419
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Description

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Antibody Name:Anti-EPHA4 Antibody
Antibody SKU:CAB8346
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 887-986 of human EPHA4 (NP_004429.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 887-986 of human EPHA4 (NP_004429.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SLKR TGTE SSRP NTAL LDPS SPEF SAVV SVGD WLQA IKMD RYKD NFTA AGYT TLEA VVHV NQED LARI GITA ITHQ NKIL SSVQ AMRT QMQQ MHGR MVPV
Gene ID:2043
Uniprot:P54764
Cellular Location:Cell junction, Cell membrane, Cell projection, Early endosome, Single-pass type I membrane protein, axon, dendrite, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:104kDa/109kDa
Observed MW:110kDa
Synonyms:EPHA4, HEK8, SEK, TYRO1
Background:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.
UniProt Protein Details:

Protein type:EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; axon; early endosome membrane; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium

Molecular Function:identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; motor axon guidance; regulation of astrocyte differentiation; positive regulation of dendrite morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior; ephrin receptor signaling pathway; corticospinal tract morphogenesis; cell adhesion

NCBI Summary:This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]
UniProt Code:P54764
NCBI GenInfo Identifier:4758280
NCBI Gene ID:2043
NCBI Accession:NP_004429.1
UniProt Secondary Accession:P54764,Q2M380,
UniProt Related Accession:P54764
Molecular Weight:109,860 Da
NCBI Full Name:ephrin type-A receptor 4
NCBI Synonym Full Names:EPH receptor A4
NCBI Official Symbol:EPHA4  
NCBI Official Synonym Symbols:SEK; HEK8; TYRO1  
NCBI Protein Information:ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8
UniProt Protein Name:Ephrin type-A receptor 4
UniProt Synonym Protein Names:EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK
Protein Family:Ephrin type-A receptor
UniProt Gene Name:EPHA4  
UniProt Entry Name:EPHA4_HUMAN
Anti-EPHA4 Polyclonal Antibody (CAB8346)Western blot analysis of extracts of various cell lines, using EPHA4 antibody (CAB8346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-EPHA4 Polyclonal Antibody (CAB8346)Immunofluorescence analysis of C6 cells using EPHA4 antibody (CAB8346) at dilution of 1:100. Blue: DAPI for nuclear staining.

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