The EPHA4 Antibody (CAB8346) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2769336 Gene ID 2043 Swiss Prot Synonym EK8; SEK; HEK8; TYRO1; EPHA4
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EPHA4 Antibody
SKU:
CAB8346
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EK8, SEK, HEK8, TYRO1, EPHA4
Positive Sample:
Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Cell Junction, Cell Membrane, Cell Projection, Early Endosome, Single-Pass Type I Membrane Protein, Axon, Dendrite, Postsynaptic Cell Membrane, Postsynaptic Density, Synapse.
Calculated MW:
110kDa
Observed MW:
110kDa
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2769336 Gene ID 2043 Swiss Prot Synonym EK8; SEK; HEK8; TYRO1; EPHA4
Purification Method:
Affinity purification
Gene ID:
2043
RRID:
AB_2769336
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EPHA4 Rabbit pAb (CAB8346) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of C6 cells using EPHA4 Rabbit pAb (CAB8346) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
