Anti-FKBP3 Antibody (CAB6907)

Antibody Name:	Anti-FKBP3 Antibody
Antibody SKU:	CAB6907
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human FKBP3 (NP_002004.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	HeLa, MCF-7, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human FKBP3 (NP_002004.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA VPQR AWTV EQLR SEQL PKKD IIKF LQEH GSDS FLAE HKLL GNIK NVAK TANK DHLV TAYN HLFE TKRF KGTE SISK VSEQ VKNV KLNE DKPK ETKS EETL DEGP PKYT KSVL KKGD KTNF PKKG DVVH CWYT GTLQ DGTV FDTN IQTS AKKK KNAK PLSF KVGV GKVI RGWD EALL TMSK GEKA RLEI EPEW AYGK KGQP DAKI PPNA KLTF EVEL VDID
Gene ID:	2287
Uniprot:	Q00688
Cellular Location:	Nucleus
Calculated MW:	25kDa
Observed MW:	30kDa

Synonyms:

FKBP3, FKBP-25, FKBP-3, FKBP25, PPIase

Background:

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin.

UniProt Protein Function:	FKBP3: FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins. Belongs to the FKBP-type PPIase family.
UniProt Protein Details:	Protein type:Isomerase; EC 5.2.1.8 Chromosomal Location of Human Ortholog: 14q21.2 Cellular Component: endoplasmic reticulum membrane; nucleus Molecular Function:protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; receptor activity Biological Process: protein peptidyl-prolyl isomerization
NCBI Summary:	The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. [provided by RefSeq, Sep 2008]
UniProt Code:	Q00688
NCBI GenInfo Identifier:	232096
NCBI Gene ID:	2287
NCBI Accession:	Q00688.1
UniProt Secondary Accession:	Q00688,Q14317, B2R4Q9,
UniProt Related Accession:	Q00688
Molecular Weight:	224
NCBI Full Name:	Peptidyl-prolyl cis-trans isomerase FKBP3
NCBI Synonym Full Names:	FK506 binding protein 3, 25kDa
NCBI Official Symbol:	FKBP3
NCBI Official Synonym Symbols:	FKBP-3; FKBP25; PPIase; FKBP-25
NCBI Protein Information:	peptidyl-prolyl cis-trans isomerase FKBP3; rotamase; 25 kDa FKBP; PPIase FKBP3; immunophilin FKBP25; rapamycin binding protein; 25 kDa FK506-binding protein; FK506-binding protein 3 (25kD); FK506-binding protein 25, T-cell; rapamycin-selective 25 kDa immunophilin
UniProt Protein Name:	Peptidyl-prolyl cis-trans isomerase FKBP3
UniProt Synonym Protein Names:	25 kDa FK506-binding protein; 25 kDa FKBP; FKBP-25; FK506-binding protein 3; FKBP-3; Immunophilin FKBP25; Rapamycin-selective 25 kDa immunophilin; Rotamase
Protein Family:	FK506-binding protein
UniProt Gene Name:	FKBP3
UniProt Entry Name:	FKBP3_HUMAN

	Western blot analysis of extracts of various cell lines, using FKBP3 antibody (CAB6907) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using FKBP3 antibody (CAB6907). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug FKBP3 antibody (CAB6907). Western blot was performed from the immunoprecipitate using FKBP3 antibody (CAB6907) at a dilition of 1:1000.

Antibodies

FKBP3 Antibody (PACO09305)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)