Western blot analysis of Rat liver, using GAS2 Rabbit mAb (CAB23552) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The GAS2 Monoclonal Antibody (CAB23552) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed and validated by Assaygenie, is raised in mice and specifically targets the GAS2 protein, allowing for precise detection and analysis in various experimental settings.GAS2 is known for its role in regulating actin dynamics and microtubule stability, making it essential for processes such as cell migration, division, and shape maintenance. By targeting GAS2 with this monoclonal antibody, researchers can gain insights into the mechanisms underlying these cellular functions and potentially uncover new therapeutic targets for diseases such as cancer and neurological disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GAS2 Monoclonal Antibody
SKU:
CAB23552
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC3102
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2620
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat liver, using GAS2 Rabbit mAb (CAB23552) at 1:900 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
Western blot analysis of various lysates using GAS2 Rabbit mAb (CAB23552) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s.
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