Anti-PIST/GOPC Antibody (CAB0582)

Product Name:	PIST/GOPC Rabbit mAb
Product Code:	CAB0582
Size:	20uL, 50uL, 100uL
Synonyms:	CAL, FIG, GOPC1, PIST, dJ94G16.2
Applications:	WB, IHC, IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human PIST/GOPC

Applications:	WB, IHC, IF
Recommended Dilutions:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, U-87MG, Rat brain, Mouse lung

Immunogen:	A synthesized peptide derived from human PIST/GOPC
Purification Method:	Affinity purification
Storage:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	57120
Uniprot:	Q9HD26
Cellular Location:	Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein, dendrite, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network membrane
Calculated MW:	51kDa
Observed MW:	59kDa

UniProt Protein Function:	PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.
UniProt Protein Details:	Protein type:Membrane protein, peripheral Chromosomal Location of Human Ortholog: 6q21 Cellular Component: Golgi membrane; Golgi apparatus; postsynaptic membrane; trans-Golgi network transport vesicle; protein complex; membrane; postsynaptic density; cytoplasm; dendrite; plasma membrane; cell junction Molecular Function:protein C-terminus binding; protein binding; protein homodimerization activity; frizzled binding Biological Process: Golgi to plasma membrane transport; protein transport; ER to Golgi vesicle-mediated transport; apical protein localization; regulation of catalytic activity; spermatid nuclear differentiation; protein homooligomerization; cytoplasmic sequestering of CFTR protein Disease: Spermatogenic Failure 6
NCBI Summary:	This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9HD26
NCBI GenInfo Identifier:	74762751
NCBI Gene ID:	57120
NCBI Accession:	Q9HD26.1
UniProt Secondary Accession:	Q9HD26,Q59FS4, Q969U8, A6NM30,
UniProt Related Accession:	Q9HD26
Molecular Weight:	462
NCBI Full Name:	Golgi-associated PDZ and coiled-coil motif-containing protein
NCBI Synonym Full Names:	golgi-associated PDZ and coiled-coil motif containing
NCBI Official Symbol:	GOPC
NCBI Official Synonym Symbols:	CAL; FIG; PIST; GOPC1; dJ94G16.2
NCBI Protein Information:	Golgi-associated PDZ and coiled-coil motif-containing protein; dJ94G16.2 PIST; fused in glioblastoma; CFTR-associated ligand; PDZ protein interacting specifically with TC10; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated PDZ and coiled-coil motif containing protein; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
UniProt Protein Name:	Golgi-associated PDZ and coiled-coil motif-containing protein
UniProt Synonym Protein Names:	CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10; PIST
Protein Family:	Golgi-associated PDZ and coiled-coil motif-containing protein
UniProt Gene Name:	GOPC
UniProt Entry Name:	GOPC_HUMAN